目的：检测维吾尔族人群人类抗原R (human antigen R, HuR)蛋白在正常胃黏膜和胃癌组织中的表达，分析其与胃癌临床病理因素的关系。 方法: 从喀什地区第一人民医院收集胃癌组织蜡块标本30例和对应癌旁组织蜡块标本23例，采用组织芯片和免疫组化SP法检测HuR在胃癌和癌旁组织中的表达；电化学发光法检测胃癌患者血清中AFP、CEA、CA199、SCC的含量；分析HuR表达与胃癌患者临床病理参数及肿瘤标志物表达水平的关系。 结果: HuR在胃癌组织细胞的胞质和胞核中均有表达，胞核中HuR在胃癌组织中的表达阳性率为83.33%，显著高于癌旁组织的52.17%（P<0.05），但其表达水平与胃癌临床病理分期无相关性；胞质中HuR在胃癌组织中表达阳性率为70%，显著高于癌旁组织的34.78%（P<0.05），其表达水平与淋巴结转移和临床分期相关（P<0.05），并且与肿瘤标志物CA199相关（P<0.05）。 结论： 在维吾尔族人群胃癌中，HuR在胃癌细胞胞核和胞质中的表达均显著高于癌旁组织；且胞质中HuR表达与淋巴结转移、临床分期以及标志物CA199密切相关。

Objective: To detect the expression of HuR (human antigen R) protein in normal gastric mucosa and gastric cancer tissues of Uygur population, and to analyze the relationship between HuR protein and clinicopatholog-ical factors of gastric cancer. Methods: Thirty cases of paraffin embedded gastric tissue samples and 23 cases of cor-responding normal gastric mucosa specimens from First People's Hospital of Kashgar region were collected for this study. The expression levels of HuR in gastric cancer tissues and normal gastric mucosa tissues were examined by tissue microarray and immunohistochemistry SP method; concentrations of AFP, CEA, CA199 and SCC in serum of the patients with gastric cancer were examined by electrochemical luminescence assay. The relationship between HuR expression and clinicopathological characteristics as well as expression of tumor markers in gastric cancer pa-tients were analyzed. Results: HuR was expressed both in the cytoplasm and nucleus of the gastric cancer cells. The positive expression of HuR in nucleus of gastric cancer cells was 83.33%, which was significantly higher than that in normal gastric mucosa cell (52.17%, P<0.05); However, the protein expression was not correlated with the clini-copathological stage of gastric cancer. HuR expression in cytoplasm of gastric cancer cells was 70%, which was sig-nificantly higher than that in normal gastric mucosa (34.78%, (P<0.05), and was correlated with lymph node metas-tasis and clinical stage (P<0.05); In addition, its expression was associated with tumor marker CA199 (P<0.05). Conclusion: In Uygur population, the expression of HuR is highly expressed in the nuclei and cytoplasm of gastric cancer cells, which is significantly higher than that in normal gastric mucosa cells. Moreover, HuR expression in cy-toplasm is associated with lynphonode metastasis, clinical staging and tumor marker CA199.

新疆生产建设兵团应用基础研究资助项目(No. 2015AG015)