目的:分析肺腺癌组织中RNA结合基序蛋白38基因（RNA-binding motif protein 38, RBM38）及抑癌基因p53 mRNA和蛋白的表达情况，探讨其在肺腺癌发生发展中的意义。 方法 : 取2012年10月至2015年6月新疆医科大学附属肿瘤医院收治的50例肺腺癌患者的肿瘤组织标本作为实验组，相对应的癌旁组织标本作为对照组。用RT-PCR法检测两组中RBM38及p53mRNA相对表达量，用Western blotting法检测两组中RBM38及p53蛋白的相对表达量。 结果 : 实验组RBM38 mRNA及蛋白相对表达量（0.357±0.170、0.294±0.149）均高于对照组（0.271±0.128、0.206±0.099），实验组p53 mRNA及蛋白（0.457±0.208、0.671±0.200）相对表达量均高于对照组（0.308±0.167、0.332±0.071），差异均有统计学意义（均 P<0.01）。RBM38 表达与肺腺癌患者 TNM 分期、浸润深度有关(P<0.05)，p53 表达与患者TNM分期有关(P<0.05)。实验组 RBM38与p53蛋白表达呈负相关（r=-0.626，P<0.01)。 结论: 肺腺癌组织RBM38、p53 mRNA及蛋白表达均高于癌旁组织，两者均与患者TNM分期等病理参数关系密切。随着RBM38蛋白表达的增加p53蛋白随之减少，RBM38可能通过抑制p53的翻译从而促进肺癌的发生发展，RBM38可能成为肺腺癌分子靶向治疗的靶点。

Objective: To observe the mRNA and protein expressions of RNA-binding motif protein 38 (RBM38 or RNPC1) and tumor suppressor gene p53 in lung adenocarcinoma tissues, and to explore its significance in the occur-rence and development of lung adenocarcinoma. Methods: Tumor tissue samples from 50 lung adenocarcinoma pa-tients that treated in Affiliated Tumor Hospital of Xinjiang Medical University were selected as the experiment group, and the corresponding adjacent tissue samples were taken as the control group. The relative mRNA expres-sion of RBM38 and p53 in two groups were detected by RT-PCR method, and the relative protein expression of RBM38 and p53 were detected by Western blotting. Results: The mRNA and protein expressions of of RBM38 in the experimental group (0.357±0.170, 0.294±0.149) were higher than those of the control group (0.271±0.128,0.206±0.099), and the mRNA and protein expressions of p53 (0.457±0.208, 0.671±0.200) were higher than those of control group (0.308±0.167, 0.332±0.071); The difference was statistically significant (all P < 0.01). RBM38 expres-sion was related to TNM stage and depth of invasion in patients with lung adenocarcinoma (P < 0.05). The expres-sion of p53 was related to the TNM staging of the patients (P<0.05). The expression of RBM38 and p53 protein in the experience group were correlated (r=- 0.626, P<0.01). Conclusion: The mRNA and protein expressions of RBM38 and p53 in lung adenocarcinoma tissues were higher than that in adjacent tissues, and they were closely re-lated to the pathological parameters of the patients, such as TNM staging. With the increase of RBM38 protein ex-pression and decrease of p53 protein, RBM38 may promote the occurrence and development of lung cancer by in-hibiting the translation of p53. RBM38 may be the target of molecular targeted therapy for lung adenocarcinoma.

国家自然科学基金资助项目（No. 81460354)）