目的： 探讨miR-223调控急性淋巴细胞白血病（acute lymphoblastic leukemia，ALL）E6-1细胞的增殖和体内成瘤能力及其作用机制。 方法： 应用实时荧光定量PCR检测miR-223在不同ALL细胞株中的表达水平，免疫荧光染色法检测携带miR-223mimic慢病毒感染E6-1细胞株的感染效率，双荧光素酶实验检测miR-223和Lmo2的相互结合关系，MTT增殖实验和克隆形成实验检测过表达miR-223对E6-1细胞株增殖和克隆形成能力的影响。裸鼠体内成瘤实验检测miR-223过表达对E6-1细胞成瘤能力的影响。Western blotting检测miR-223对MAPK信号通路相关蛋白表达的影响。 结果： miR-223在E6-1细胞株中表达水平相对较低（P<0.05），双荧光素酶实验证实miR-223可以直接靶向结合Lmo2的3’UTR区序列。过表达miR-223后： （1）抑制E6-1细胞的增殖能力（0.16±0.02 vs 1.15±0.21，P<0.05）； （2）抑制E6-1细胞的裸鼠体内成瘤能力[ （0.56±0.08）vs（1.69±0.22）g，P<0.05]； （3）调控MAPK信号通路的活性。 结论： miR-223可靶向作用Lmo2通过MAPK信号通路调控ALL细胞的增殖、克隆形成和体内成瘤能力。

Objective: To investigate whether the expression of Toll like receptor-4 (TLR-4) has an effect on the phenotypes and function of dendritic cells (DCs) and the possible mechanism. Methods: pcDNA3.1 plasmid encod-ing full TLR-4 gene sequence was constructed as a template to obtain TLR-4 mRNA through in vitro transcription us-ing mMESSAGE mMACHINE T7 Kit; DCs from healthy human peripheral blood were transfected with TLR-4 mRNA by liposomal transfection. The functional molecule expression on DC surface and the ability of DC inducing cytotoxic T lymphocytes (CTLs) to secrete cytokines in vitro were detected by Flow cytometry after transfection.Results: Human TLR-4-pcDNA3.1 plasmid was successfully constructed; human TLR-4 and EGFP mRNA frag-ments were successfully amplified. The Flow cytometry results showed that the expressions of chemokine CCR7 and functional molecule HLA-DR on dendritic cell surface were significantly increased after transfection with TLR-4 mRNA compared with control mRNA transfection or pre-ransfection (CCR7: [42.4±4.93]% vs [20.1±3.09]%，[17.1±4.33]%，P<0.05; HLA-DR:[62.1±7.23]% vs [17.7±6.01]%，25.8±4.16]%，P<0.05); and the ability of secret-ing IFN-γ from CTLs induced by TLR-4 mRNA-DCS was significantly enhanced in vitro compared with control mRNA-DC，empty-DC and PBMC ([66.5±3.58]% vs [41.1±4.27]%，[37.9±2.96]%，[3.2±2.03]%，P<0.05). Conclu-sion: The function of dendritic cells could be significantly enhanced by TLR-4 mRNAtransfection. The ability of an-tigen presentation and inducing CTLs of dendritic cell were improved, which would provide an experimental basis for enhancing the anti-tumor effect of DC vaccines.

国家临床重点专科建设项目资助；福建省科技计划项目资助（No.2017Y0022）；福建省自然基金项目资助（No.2016J01514）