目的: 探讨Testin基因（TES）在非小细胞肺癌（NSCLC）组织和细胞株中的表达及其对人肺癌A549细胞增殖、迁移、侵袭和凋亡的影响。 方法 : 收集2015年1月至2015年12月在华中科技大学同济医学院附属同济医院手术切除的27例NSCLC患者的癌组织及癌旁组织标本，用Western blotting法检测癌组织和癌旁组织，以及正常人胚肺成纤维细胞株MRC5和肺癌细胞株A427、A549、H1299、LK2、PC9和SW900中TES蛋白的表达水平。应用短发卡RNA（shRNA）瞬时转染肺癌细胞株A549干扰TES基因的表达，并进一步检测TES低表达对A549细胞增殖、迁移、侵袭以及凋亡的影响，同时检测凋亡相关蛋白Bax、Bcl-2和Cas-pase-3的表达。 结果： 在NSCLC组织和细胞株中TES蛋白的表达明显下降（均P<0.05）。shTES干扰A549细胞后，TES mRNA和蛋白表达水平均显著下降（均P<0.05）。抑制TES表达显著增强A549细胞的增殖[(2.75±0.04) vs (1.79±0.06), P<0.05]、迁移［(52.3±2.6)% vs(19.7±1.4)%, P<0.05］和侵袭能力［(31.2±3.9) % vs (14.5±4.1)%, P<0.05］，同时降低了细胞凋亡率［(8.2±1.1)% vs(23.1±1.7)%, P<0.05］。TES 低表达使 A549 细胞 Bax 和 Caspase-3 蛋白表达明显下降（P<0.05）、Bcl-2 蛋白表达明显升高（P<0.05）。 结论： TES在NSCLC组织中呈低表达，TES表达下调具有促进肺癌细胞的增殖、迁移、侵袭并抑制凋亡等生物学效应，其有可能成为肺癌治疗一个新靶点。

Objective: To investigate the expression of Testin (TES) gene in non-small lung cancer cell (NSCLC) lines, and also to investigate its effect on cell proliferation, migration, invasion and apoptosis of human lung cancer cell line A549. Methods: Twenty-seven cases of cancer tissues and corresponding para-cancerous tissues from non-small lung cancer patients that treated in Tongji Hospital Affiliated to Tonji Medical College of Huazhong Universi-ty of Science and Technology between January 2015 and December 2015 were collected for this study; and the ex-pression of TES protein in collected tissues was examined by Western blotting. In the meanwhile,TES protein ex-pressions in six human lung cancer cell lines (A427, A549, H1299, LK2, PC9 and SW900) and lung fiberblast cell line MRC5 were also detected. shTES was transiently transfected into A549 cells to interfere the expression of TES gene; the effect of low TES expression on proliferation, migration, invasion and apoptosis of A549 were further as-sessed, and its effect on the expressions of apoptosis related proteins (Bax, Bcl-2 and Caspase-3) were also tested by Western blotting. Results: The expression of TES protein in lung cancer tissues and lung cancer cell lines was signif-icantly decreased (all P<0.05). After shTES transfection in A549 cells, the expressions of TES mRNA and protein were significantly down-regulated (P<0.05). Additionally, shTES significantly increased cell proliferation ([2.75±0.04］vs [1.79±0.06], P<0.05), migration ([52.3±2.6]% vs [19.7±1.4]%, P<0.05), and invasion ([31.2±3.89]% vs [14.5±4.1]%, P<0.05), while inhibited cell apoptosis ([8.2±1.1]% vs [23.1±1.7]%, P<0.05) of A549 cells. Moreover,TES low expression significantly decreased the expressions of Bax and Caspase-3 (P<0.05) but increased the expres-sion of Bcl-2 (P<0.05) in A549 cells. Conclusion:TES was low expressed in NSCLC tissues, and down-regulation of TES could promote cell proliferation, migration, invasion and inhibit the apoptosis of lung cancer cells, which may serve as a new target for lung cancer treatment.