目的：探讨长链非编码RNA（long non-coding RNA, lncRNA）肺癌转移相关转录本1 (metastasis-associated lung ade-nocarcinoma transcript 1, MALAT1)在弥漫性大B细胞淋巴瘤(diffuse large B-cell lymphoma, DLBCL)患者血清中的表达水平及其与临床预后的相关性。 方法： 采集2010年1月至2015年12月期间在四川省人民医院血液科确诊的、临床资料完整的82例DL-BCL患者和32例淋巴结反应性增生（reactive lymphoid hyperplasia, RLH）患者的血液标本，用实时荧光定量PCR法检测两组患者血清中lncRNAMALAT1的表达水平，分析lncRNAMALAT1表达水平与DLBCL患者临床病理特征及预后的关系。 结果: DLB-CL患者血清中lncRNA MALAT1表达水平明显高于RLH患者（7.48±0.27 vs 1.28±0.45，P<0.01）。lncRNA MALAT1的表达水平与肿瘤大小、临床分期、B症状及国际预后指数（international prognostic index，IPI）相关(均P<0.01)。高表达lncRNA MALAT1的患者中位无进展生存时间明显短于低表达者[(16.43±2.05) vs (32.01±3.20)个月，P<0.01]，lncRNA MALAT1表达水平与IPI指数是影响DLBCL患者预后的独立因素(P<0.01)。 结论: lncRNA MALAT1在DLBCL患者中的高表达与多项临床病理参数有关，有望成为判断DLBCL患者预后的标志物。

Objective: To investigate the expression of lncRNAMALAT1 (long non-coding RNAs, metastasis-asso-ciated lung adenocarcinoma transcript 1) in the serum of DLBCL (diffuse large B-cell lymphoma) patients, and to analyze its correlation to clinical prognosis. Methods: The blood samples of 82 DLBCL patients and 32 RLH patients (lymph node reactive hyperplasia), who were diagnosed at Hematology Department of People’s Hospital of Sichuan Province from January 2010 to December 2015 with complete clinical data, were collected for this study. ln-cRNA MALAT1 expression was detected by Real-time fluorescence quantitative PCR, and the relationship between lncRNA MALAT1 expression with clinical pathological features and prognosis were analyzed. Results: Compare with RLH patients, lncRNA MALAT1 expression was significantly increased in DLBCL patients (7.48±0.27 vs 1.28±0.45, P<0.01). In addition, lncRNA MALAT1 was significantly correlated with tumor size, clinical stage, B symptoms and international prognostic index (IPI) scores. The median progression free survival time (PFS) of patients with high lncRNA MALAT1 expression was significantly shorter than those with low expression ([16.43±2.05] months vs [32.01±3.20] months, P<0.01). Cox multivariate analyses verified that lncRNA MALAT1 and IPI scores were independent predictive factors for DLBCL prognosis. Conclusion: The high expression of lncRNA MALAT1 in DLBCL patients was correlated with multiple clinicopathological parameters., and it is expected to become a new tumor marker for DLBCL prognosis.

四川省卫生和计划生育委员会科技资助项目（No.16PJ475）