目的：探讨香加皮杠柳苷（cortex periplocae, CPP）联用肿瘤坏死因子相关凋亡诱导配体（tumor necrosis factor related apoptosis inducing ligand，TRAIL）对胃癌细胞的作用及其机制。 方法： 人胃癌细胞系SGC-7901、MGC-803培养完成后，采用50、100、200 ng/ml的CPP和1 μg/ml的TRAIL单用或联合处理。MTS法检测SGC-7901和MGC-803细胞的增殖情况，流式细胞术检测其凋亡率，Western boltting检测pro-BID、Mcl-1、cleaved caspase-3、DR4、DR5的表达水平。 结果： SGC-7901和MGC-803细胞经CPP（50、100、200 ng/ml）和TRAIL（1 μg/ml）联合处理24 h后，细胞增殖率明显低于空白对照组和对应的CPP各剂量单独处理组（ P <0.05或 P <0.01）。SGC-7901和MGC-803细胞凋亡率均明显高于空白对照组和对应的CPP各剂量单独处理组（P<0.05或P<0.01）。SGC-7901和MGC-803细胞中pro-BID、Mcl-1表达水平明显降低（均 P <0.05），cleaved caspase-3表达水平明显升高（ P <0.05），DR4和DR5受体的表达水平升高（均P<0.05）。 结论： CPP协同TRAIL可明显诱导人胃癌SGC-7901和MGC-803细胞凋亡，增强人胃癌细胞对TRAIL的敏感性。

Objective:To investigate the effect of combined treatment of cortex periplocin (CPP) and tumor necro-sis factor related apoptosis inducing ligand (TRAIL) on gastric cancer cells and to explore the mechanism. Meth-ods: After routine culture, the gastric cancer cell lines (SGC-7901 and MGC-803) were treated with CPP (at concen-trations of 50，100，200 ng/ml) or TRAIL (1 μg/ml) or in combination of these two. The cell proliferation was detect-ed by MTS, the apoptosis was detected by Flow cytometry, and the expression levels of pro-BID，Mcl-1，cleaved caspase-3，DR4 and DR5 were detected by Western blotting. Results: Compared with the control group and each CPP single treatment group, MTS assay demonstrated that CPP (50，100，200 ng/ml) in combination with TRAIL (1 μg/ml) significantly inhibited the proliferation of gastric cancer SGC-7901 and MGC-803 cell lines (all P<0.05 or P<0.01). Flow cytometry demonstrated that the apoptosis rate of gastric cancer cells in combined treatment group was significantly higher than that of control group or each CPP single treatment group (P<0.05 or P<0.01). Western blotting demonstrated that combined treatment for 24 h significantly decreased the expression of pro-BID and Mcl-1 (P<0.05), but increased the expression levels of cleaved caspase-3，DR4 and DR5(P<0.05). Conclusion: CPP in combination with TRAIL could significantly induce the apoptosis of gastric cancer SGC-7901 and MGC-803 cell lines and increase the susceptibility of cancer cells to TRAIL.

国家科学自然基金项目资助（No.81673642)