目的：检测miR-141-3p在胃癌组织及患者血浆中的表达水平，探讨其表达水平与患者病理特征和预后的关系。 方法： 收集河北医科大学第四医院普外科2012年5月至2013年1月胃癌根治性手术切除的组织标本及术前外周静脉血标本44例，每例标本采集肿瘤组织(非坏死部分)和配对的癌旁组织。采用实时荧光定量PCR法检测miR-141-3p在胃癌组织、癌旁组织、血浆及健康志愿者血浆（25例）的表达情况，分析miR-141-3p的表达水平与胃癌患者DFS及临床病理特征的关系。 结果： miR-141-3p在胃癌组织中的表达明显降低，在血浆中的表达水平明显升高（均P<0.01）。在胃癌组织中miR-141-3p高表达组DFS明显高于低表达组（21.8 vs 10.3个月, P<0.01），且miR-141-3p在癌组织中的表达水平与患者DFS呈正相关（P<0.01）。在血浆中miR-141-3p的高表达组DFS明显低于低表达组（9.1 vs 21.0个月,P<0.01）,且miR-141-3p血浆中的表达水平与患者DFS呈负相关（P<0.01）。miR-141-3p在胃癌患者血浆中表达水平与肿瘤TNM分期、淋巴结转移及脉管瘤栓相关（P<0.01）。 结论： miR-141-3p在胃癌组织中表达显著降低且与患者预后呈正相关，其在患者血浆中表达显著升高且与患者预后呈负相关，表明miR-141-3p可作为胃癌早诊早治及患者临床预后判定的潜在标志物。

Objective:To investigate the relationship between miR-141-3p expression and prognosis and the clini-cal parameters of gastric cancer patients through detecting the expression of miR-141-3p in gastric cancer tissues and plasma. Methods: Tissue specimens and preoperative periphery venous blood specimens from 44 gastric cancer patients were collected during radical resection of gastric cancer at the Fourth Hospital of Hebei Medical University from May 2012 to January 2013; each tissue sample included cancer tissue (non-necrosis part) and the correspond-ing non-cancerous tissue. qRT-PCR was adopted to detect the expression of miR-141-3p in gastric cancer tissues,normal para-cancerous tissues and plasma of gastric cancer patients as well as healthy volunteers (25 cases). The re-lationship between miR-141-3p expression and DFS and clinical parameters were also investigated. Results: miR-141-3p was dramatically decreased in gastric cancer tissues but significantly increased in plasma (all P<0.01). In re-gard of tissue sample examination, DFS of high miR-141-3p expression group was significantly longer than that of low expression group (21.8 vs 10.3 months, P<0.01), and miR-141-3p expression was positively correlated with DFS of patients (P<0.01). In regard of plasma sample examination, DFS of high miR-141-3p expression group was significantly shorter than that of low expression group (9.1 vs 21.0 months, P<0.01), and miR-141-3p expression was negatively correlated with DFS (P<0.01). The plasma miR-141-3p expression was associated with TNM stage,lymph node metastasis and venous obstruction (P<0.01). Conclusion: miR-141-3p was dramatically decreased in gastric cancer tissues and positively correlated with the prognosis; while its expression was significantly increased in plasma and negatively correlated with prognosis of patients, indicating that miR-141-3p could serve as a potential target for the timely treatment and the prognosis prediction of gastric cancer.

河北省卫计委医学科学研究基金资助项目（No. 20170150）;河北省科技计划资助项目（No.162777138）