目的：探索骨膜蛋白（periostin, POSTN）在乳腺癌组织、癌旁组织、乳腺正常组织中的表达情况及其临床意义。方法：选取昆明医科大学第三附属医院乳腺科2012 年5 月至2016 年12 月手术切除的45 例乳腺癌患者的癌组织、癌旁组织及正常乳腺组织，另取15 例患者手术切除的无菌癌组织及正常组织进行原代成纤维细胞培养，H-E及免疫组化染色鉴定原代培养的成纤维细胞。采用实时定量PCR（qPCR）分别分析乳腺癌组织、癌旁组织和乳腺正常组织以及癌相关成纤维细胞和正常成纤维细胞中POSTN mRNA的表达情况，并用相关性分析乳腺癌组织中POSTN表达水平和乳腺癌患者临床病理特征的关系。乳腺癌组织芯片H-E及免疫组化染色检测POSTN蛋白在乳腺癌组织中的表达定位。结果：乳腺癌组织POSTN mRNA表达水平显著高于癌旁组织和正常乳腺组织（P<0.05）；癌相关成纤维细胞POSTN mRNA表达与正常成纤维细胞无明显差异（P>0.05）。POSTN蛋白主要在乳腺癌实质组织中表达，间质中表达较少。乳腺癌组织中POSTN mRNA表达水平与乳腺癌患者雌激素受体呈负相关（P<0.05)。结论：POSTN主要在乳腺癌组织实质中表达，其在乳腺癌组织中高表达可能与乳腺癌的发生发展相关。

Objective: To explore expression status of periostin (POSTN) in the mammary cancer, para-cancer and normal mammary tissues and its clinical significance. Methods: The mammary cancer, para- cancer and normal mammary tissues of 45 patients with mammary cancer，which were taken out by exairesis in Department of Galactophore,Third Affiliated Hospital of Kunming Medical University during May 2012 to December 2016. In addition,from the tissues fifteen amicrobic mammary cancer and normal mammary tissues were selected for primary cell culture.The primary cultured cells were identified by HE staining and immunohistochemistry assays. Expression status of POSTN mRNA in the mammary cancer, para-cancer and normal mammary tissues as well as in the cancer associated fibroblast (CAF) and normal fibroblast (NF) were detected by real-time quality PCR (qPCR). Correlation analysis was used to analyze the relationship between expression level of POSTN in the mammary tissue and clinical pathological features of the patients with mammary cancer. Location of POSTN protein in the mammary cancer tissue was identified by HE staining and immunohistochemistry of the mammary cancer tissue chips. Results: Expression level of POSTN mRNA in the mammary cancer tissue was significantly higher than those in the para-cancer and normal mammary tissues (P<0.05), expression level of POSTN mRNA was similar between CAF and NF (P>0.05).POSTN protein mainly expressed in substances of the mammary cancer tissues and less expressed in the mesenchyma of the mammary cancer tissue. Expression level of POSTN mRNA in the mammary cancer tissue was negatively correlated to estrogen receptor (ER) of the patients with mammary cancer (P<0.05). Conclusion: POSTN was mainly expressed in substances of the mammary cancer tissues, high expression of which in the mammary tissues could be related to occurrence and development of the mammary cancer.

国家自然科学基金资助项目（No.81460358)，云南省科技厅—昆明医科大学应用基础研究联合专项资助[No. 2017FE467(-189)，2014FB066]；云南省卫计委科技计划资助项目（No.2017NS201, 2014NS023）