目的：探讨细胞外基质蛋白spondin2(SPON2)在人胃癌组织中的表达及其与胃癌发生的关系。方法：收集2014 年6月至2016 年7 月在苏州大学附属张家港医院行胃癌根治术75 例患者的胃腺癌和相应癌旁组织，应用免疫荧光化学染色观察SPON2 蛋白在胃癌及癌旁组织的细胞定位,应用免疫组化染色检测SPON2 蛋白在胃腺癌及癌旁组织中的表达，分析SPON2 蛋白表达与胃癌患者临床病理特征的关系。结果：SPON2 蛋白定位于胃癌细胞的胞膜、胞质及胞外基质中，SPON2 蛋白在胃癌组织中的表达明显高于癌旁组织（72.0% vs 16.0%,P<0.01)。SPON2 蛋白表达与肿瘤细胞分化程度以及患者的性别、年龄无关，与胃癌肿块大小、TNM分期、浸润程度、淋巴结转移有关(均P<0.05)。结论：SPON2 蛋白在胃癌组织中的高表达与胃癌的发生发展有关，其有可能作为新的胃癌早期诊治靶点，并可能预测患者的预后及转归。

Objective: To explore expression of extracellular matrix protein spondin2 (SPON2) in human gastric carcinoma tissue and its relationship with occurrence of the gastric carcinoma. Methods: Gastric adenocarcinoma and para-carcinoma tissues of 75 patients who hospitalized in Zhangjiagang Hospital affiliated to Suzhou University for eradication of the gastric carcinoma during June 2014 to July 2016 were collected. Immunoflorescence staining was used to survey localization of SPON2 protein in cells of the gastric carcinoma and the para-carcinoma tissues.Expressions of SPON2 in the gastric carcinoma and the para-carcinoma tissues were detected by immunohistochemical staining. Relationship between expression of SPON2 protein and clinicopathological features of the patients with gastric carcinoma was analyzed. Results: SPON2 protein was localized in cytomembrane, cytoplasm and extracellular matrix of the gastric carcinoma cells. Expression of SPON2 protein in the gastric carcinoma tissue was significantly higher than that in the para-carcinoma tissue (72.0% vs 16.0%, P<0.01). There was no relationship between expression of SPON2 protein and tumor cell differentiation, gender, age of the patients with gastric carcinoma, as well as there were significant relationships between expression of SPON2 protein and tumor size, TNM staging, invasion degree, lymph node metastasis (all P<0.05). Conclusion: High expression of SPON2 protein in the gastric carcinoma tissue was related to occurrence and development of the gastric carcinoma, which suggests that SPON2 protein might become a novel marker of early clinical diagnosis and a potential therapeutic target of the gas-tric carcinoma as well as could forecast prognosis of the patients with gastric carcinoma.

苏州市医学重点诊疗项目资助（No.LCZX201617）