目的：研究核内不均一性核糖核蛋白A1 基因(heterogeneous nuclear ribonucleoproteins A1, hnRNP A1) mRNA水平在消化系统肿瘤组织中的表达及其临床意义。方法：利用TCGA数据库全基因组mRNA测序资料分析hnRNPA1 在消化道肿瘤与正常组织间表达的差异，分析其表达水平与临床病理资料的相关性，并进行生存分析判断其预后价值。结果：（1）hnRNPA1 在不同消化系统肿瘤组织中表达较正常组织均明显增高（P<0.01）,其中结直肠癌、肝癌、胆管癌、胰腺癌更为明显。（2）hnRNPA1 在肝癌组织中的表达与肿瘤分化程度(P<0.05)、TNM分期(P<0.05) 和浸润深度(P<0.05)相关，与年龄、性别、肝癌危险因素无关联。hnRNPA1 在胆管癌、胰腺癌组织中的表达与年龄、性别、TNM分期、浸润深度等不相关。（3）hnRNPA1 在胰腺癌组织中的表达高低与总生存期有相关性（P<0.05），且为独立于TNM分期、分化程度而影响患者预后的危险因子；hnRNPA1 在结肠、直肠、肝、胆管肿瘤组织中的表达高低与总生存期无相关性。结论：hnRNPA1 mRNA在不同消化系统肿瘤中表达都增高；其高表达是胰腺癌独立预后不良因素，可以作为判断预后的有效生物标志物。

Objective：To investigate the expression patterns of hnRNPA1 mRNA in the digestive system tumors and its clinical significance. Methods：Whole genome mRNA sequencing data of TCGA databases were used to analyze the different expression of hnRNPA1 in tumor and normal tissues and to study its correlation to clinical pathological data; and survival analysis was performed to determine its prognostic value. Results：1. The expressions of hnRNPA1 were significantly higher in digestive system tumor tissues than that in normal tissues（P<0.01），especially in colorectal cancer, hepatocellular carcinoma, pancreatic cancer and cholangiocarcinoma. 2. In hepatocellular carcinoma,hnRNPA1 expression was correlated with tumor differentiation, TNM stage and the depth of invasion (all P<0.05), but not associated with age, sex, risk factors of liver cancer. In pancreatic cancer and cholangiocarcinoma, the expression of hnRNPA1 was not significantly associated with age, sex, TNM stage and infiltration depth etc. 3. The expression of hnRNPA1 was correlated with overall survival in pancreatic cancer patients (P<0.05), which is a risk factor for the prognosis that independent of TNM stage, degree of differentiation; the expression of hnRNPA1 was not significantly associated with overall survival in colorectal cancer, hepatocellular carcinoma and cholangiocarcinoma.Conclusion：The expression of hnRNPA1 mRNA was increased in tumor tissues of different digestive system cancers; its over- expression is an independent risk factor for the prognosis of pancreatic cancer, which might be served as a potential biomarker for predicting prognosis.

国家自然科学基金资助项目(No.81370484）