目的: 探讨非小细胞肺癌患者外周血有核细胞miR-205-5p 的表达水平是否可作为分子标志物。方法: 收集昆明医科大学第三附属医院2011 年6 月至2012 年6 月非小细胞肺癌首诊患者60 例，其中无远处转移患者30 例，有远处转移患者30例。另外收集健康志愿者30 例作为对照组。qPCR技术检测人支气管上皮细胞BEAS-2B、云南个旧肺鳞癌细胞YTMLC-90、云南宣威肺腺癌细胞XWLC-05 以及人外周血有核细胞的表达水平。分析外周血有核细胞miR-205-5p 的表达水平与非小细胞肺癌患者血清标志物水平、TNM分期、淋巴结转移、远处转移等临床病理参数的相关性。结果：miR-205-5p 在BEAS-2B细胞中表达水平低（0.820 8±0.255 3），而在YTMLC-90（17.507 2±1.906 3）和XWLC-05（5.725 2±1.012 0）细胞中表达水平明显升高，差异有统计学意义(P<0.05)；miR-205-p 在健康人外周血中的表达水平显著低于无转移的以及转移的非小细胞肺癌患者（1.073 0 vs 3.658 8、19.6324，均P<0.01）。外周血有核细胞miR-205-5p 的阳性表达率与非小细胞肺癌患者性别、年龄、淋巴结转移、病理类型和血清肿瘤标志物水平（CEA、CA125、CA242）无关(P>0.05)，而与患者远处转移、TNM分期、血清肿瘤标志物CA153 水平明显相关(P<0.01)。结论：miR-205-5p表达水平异常升高可能作为非小细胞肺癌患者转移相关的分子标志物。

Objective: To investigate whether miR- 205- 5p expression in peripheral blood karyocytes from nonsmall cell lung cancer (NSCLC) patients could be used as a reasonable molecule biomarker. Methods: Peripheral blood from 60 patients, who were primarily diagnosed with NSCLC and treated at the Third Affiliated Hospital of Kunming Medical University (Yunnan Cancer Hospital) from June, 2011 to June, 2012, was collected; among them,there were 30 cases with distant metastasis while the other 30 without. In addition, 30 healthy subjects were enrolled as controls. The miR- 205- 5p expressions in collected peripheral blood karyocytes, human bronchial epithelial BEAS-2B cells, Gejiu squamous cell lung carcinoma YTMLC-90 cells, and Xuanwei lung adenocarcinoma XWLC-05 cells were detected by qPCR. We also analyzed the correlation between miR-205- 5p expression in peripheral blood karyocytes and NSCLC serum markers levels, TNM stage, lymph node metastasis, as well as distant metastasis etc. Results: The expressions of miR-205-5p in YTMLC-90 (17.507 2±1.906 3) and XMLC-05 cells (5.725 2±1.012 0) were significantly higher than that in BEAS-2B cells (0.820 8±0.255 3) (P<0.05). The miR-205-5p expres-sion level in peripheral blood karyocytes from healthy controls was significantly higher than that from NSCLC patients (P<0.01). The positive rate of miR-205-5p in peripheral blood karyocytes from NSCLC patients was not correlated with patients’sex, age, lymph node metastasis, pathologic subtypes and tumor serum markers (CEA，CA125,CA242) (all P>0.05), but significantly correlated with distant metastasis, TNM stage and tumor serum marker CA153 (P<0.01). Conclusion: Abnormal up-regulation of miR-205-5p may play an important role in the progression of NSCLC and can be used as a reasonable biomarker for the metastasis in NSCLC patients.

国家自然科学基金资助项目(No. 81560380, 81460358, 81060177)；云南省卫生科技项目资助（No. 2014NS023, 2014NS024）；詹启敏院士工作站基金资助（No. 2015IC002）