目的: 探讨树突状细胞-细胞因子诱导的杀伤细胞（dendritic cell-cytokine induced killer cell, DC-CIK）对结直肠癌（colorectal carcinoma, CRC）根治术后肝转移患者循环肿瘤细胞（circulating tumor cell, CTC）数量、疗效和预后的影响。方法: 回顾性分析2009 年7 月至2015 年12 月在解放军第309 医院普通外科采用DC-CIK+常规疗法治疗的CRC根治术后肝转移患者62例（DC-CIK组）和同期未接受DC-CIK治疗的70 例患者（常规组）的临床资料，同时抽取部分患者（DC-CIK组21 例，常规组24 例）的外周静脉血，用CellSearch R 免疫磁珠技术检测血中CTC的数量，分析比较两组患者的疗效和预后。结果：DC-CIK 组治疗后患者CTC数量明显低于治疗前[ （1.0±1.1）vs（2.7±2.0）个，P<0.01]，而常规组CTC变化不明显（P>0.05）；DC-CIK组肝转移灶手术切除率、治疗客观有效率、患者无进展生存和总生存均显著优于常规组（P<0.05 或P<0.01）。结论：DC-CIK可减少CRC根治术后肝转移患者CTC数量并延长患者生存时间，在规范化实施的前提下具有可靠的抗肿瘤效果。

Objective: To explore and identify the influence of dendritic cell-cytokine induced killer cell （DC-CIK）on the number of blood circulating tumor cell (CTC), therapeutic efficacy and prognosis of patients with liver metastasis after radical resection of colorectal cancer. Methods: We retrospectively analyzed the clinical data of patients developing liver metastasis after radical resection of colorectal cancer (CRC) and treated in PLA 309th Hospital between July, 2009 and December, 2015, among whom 62 patients were administered DC-CIK plus conventional treatment (DC-CIK group) and 70 received only conventional treatment (conventional group). In addition, peripheral venous blood of 21 patients in DC-CIK group and 24 patients in conventional group were examined to clarify CTC number via CellSearch R immunomagnetic technology. The therapeutic efficacy was compared between the two groups. Results: The CTC number of patients in DC-CIK group decreased significantly after treatment (1.0±1.1 vs 2.7±2.0, P<0.01), while that in conventional group did not alter obviously (P>0.05). The resection rate of liver metastasis, objective response rate to treatment, patients’progression-free survival and overall survival of DC-CIK group were all superior than those of conventional group (P<0.05 or P<0.01).Conclusion: DC-CIK can decrease the number of CTC in CRC patients with liver metastasis after radical operation and prolong their survival; it has reliable anti-tumor effect under the precondition of standard execution.

总后卫生部军事医学创新工程资助项目（No.13CXZ047）