目的:探讨姜黄素对二乙基亚硝胺( diethylnitrosamine, DEN)诱发大鼠肝细胞癌（hepatocellular carcinoma, HCC）缺氧后血管形成的影响。方法: 采用DEN诱发大鼠HCC，结扎肝动脉并构建大鼠HCC缺氧模型。将HCC模型大鼠按照数字表法随机分为单纯碘化油栓塞组（A组）、碘化油联合姜黄素栓塞组（B组）、碘化油联合肝周包膜组（C组）、碘化油联合姜黄素及肝周包膜组（D组），每组10 只。比较各组大鼠相应治疗后HCC细胞及组织VEGF、微血管密度（microvessel density，MVD）及大鼠中位生存时间（median survival time，MST）。结果：B组与D组的VEGF蛋白表达及MVD均比A组显著降低（P<0.01），而C组上述指标则与A组无显著变化（P>0.05）。B、C、D组比A组大鼠MST均显著延长（P<0.05），D组大鼠的MST高于B、C组（P<0.05）。结论：姜黄素可抑制HCC缺氧大鼠肿瘤血管的生成，可降低VEGF表达及MVD,起到延长大鼠生存期的作用。

Objective: To investigate the effect of curcumin on angiogenesis in rats with DEN (diethylnitrosamine)-induced HCC (hepatocellular carcinoma) under hypoxia. Methods: Rat HCC was induced by DEN, and its hepatic hypoxia model was established by ligating hepatic artery. The rats with established HCC model were randomly divided into four groups according to digital table method: lipiodol embolization group (group A), lipiodol combined with curcumin embolization group (group B), lipiodol combined with peripheral liver capsule group (group C), lipiodol combined with curcumin and peripheral liver capsule group (group D), with 10 rats in each group. VEGF expression in HCC cells and tissues, microvessel density (MVD), and median survival time (MST) of rats in each group were compared. Results: VEGF protein expression and microvessel density in group B, D were significantly lower than those in A group (P<0.01), while those in C group had no significant difference compared with group A (P >0.05). MST in group B, C and D was significantly longer than that in group A (P<0.05), and the MST in group D was higher than that in group B and C (P<0.05). Conclusion:Curcumin can inhibit tumor angiogenesis and decrease VEGF expression and MVD in HCC rats under hypoxia, thus further prolong the survival time of the rats.

福建省自然科学基金资助项目（No. 2017J01164）；漳州市科技拥军项目（No. ZZ2016KD06）；南京军区医学科技创新项目（No.11MA082）