目的：研究人浆细胞瘤多样异位基因1（plasma-cytoma variant translocation gene 1, PVT1）对卵巢癌SKOV3 细胞增殖、迁移和侵袭能力的影响。方法：选取2015年11 月至2017 年4 月郑州大学第三附属医院妇产科收治的卵巢癌患者32例。利用实时荧光定量PCR检测PVT1 在32例卵巢癌组织及癌旁组织的表达。通过CCK-8法、划痕法及Transwell法检测PVT1 对卵巢癌细胞增殖、迁移及侵袭能力的影响。结果：PVT1在SKOV3细胞和卵巢癌组织表达水平均明显升高（均P<0.01）；PVT1表达水平与卵巢癌者组织学分级、FIGO分期及淋巴结转移具有相关性（P<0.05或P<0.01）。转染PVT1 siRNA36、48 h 后，SKOV3细胞中PVT1 表达水平明显下降（P<0.05）；敲减PVT1的表达能够降低SKOV3细胞的增殖能力（P<0.05），抑制SKOV3细胞增殖、迁移和侵袭能力（P<0.05或P<0.01）。结论：PVT1 在卵巢癌中高表达，抑制PVT1表达能够抑制卵巢癌SKOV3细胞增殖、迁移和侵袭能力。

Objective: To investigate the effects of plasma-cytoma variant translocation gene 1 (PVT1) on proliferation, migration and invasion of ovarian cancer SKOV3 cells. Methods: Quantitative reverse transcription PCR (qRT-PCR) was used to detect the expression of PVT1 in 32 pairs of ovarian cancer tissues and corresponding para-carcinoma tissues. The effects of PVT1 on proliferation, migration and invasion of ovarian cancer cells were studied by CCK-8, scratch wound healing assay and Transwell assay. Results: The expression level of PVT1 in SKOV3 cells and ovarian cancer tissues was significantly increased (all P<0.01). The expression level of PVT1 was correlated with histological grade, FIGO stage and lymph node metastasis in patients with ovarian cancer (P<0.05 or P<0.01). After transfection with PVT1 siRNA for 36, 48 h, expression of PVT1 was significantly decreased in SKOV3 cells; and the inhibition of PVT1 expression could decrease the proliferation ability and inhibit the migration and invasion of SKOV3 cells (P<0.05 or 0.01). Conclusion: LncRNA PVT1 was highly expressed in ovarian cancer. Down-regulation of PVT1 could inhibit the proliferation,migration and invasion of ovarian cancer SKOV3 cells.

河南省科技厅科技攻关资助项目（No.162102310131）