目的： 探讨Tim-4促进子宫颈癌细胞侵袭与迁移及其相关机制。 方法： 用实时荧光定量PCR法检测子宫颈癌细胞系 Siha、Hela与子宫颈上皮永生化细胞系H8中Tim-4的表达水平。在较低表达Tim-4的Siha细胞系感染携Tim-4的慢病毒载体，荧 光显微镜观测Siha细胞系转染Tim-4的表达水平。用Transwell及划痕实验检测Tim-4对子宫颈癌细胞系Siha的侵袭与迁转移能 力的影响，用Western blotting检测Siha细胞转染Tim-4后MMP2、MMP9、E-cadherin、N-cadherin的变化。 结果： 子宫颈癌细胞系 Siha、Hela与宫颈上皮永生化细胞系H8相比高表达Tim-4。成功构建携Tim-4慢病毒载体的Siha细胞系；Tim-4明显抑制子宫颈 癌细胞系Siha的迁移与侵袭能力，且影响了MMP2、MMP9及N-cadherin、E-cadherin的表达水平。 结论： 过表达Tim-4可促进子 宫颈癌细胞的侵袭与迁移能力，其机制可能与Tim-4促进Siha细胞EMT转化有关。

Objective: To investigate the effect of Tim-4 on invasion and migration of cervical cancer cells and its underlying mechanisms. Methods：The expression levels of Tim-4 in cervical cancer cell lines Siha, Hela and cervical epithelial immortalized cell line H8 were detected by Real-time PCR. The Tim-4 lentiviral vector was transfected into Siha cell line. The over-expression of Tim-4 in Siha cell line was detected by fluorescence microscopy. The effects of Tim-4 on the invasion and migration of cervical cancer cell line were detected by Transwell and scratches methods. The changes of MMP2, MMP9, E-cadherin and N-cadherin in Siha cells were detected by Western blotting. Results：The expression of Tim-4 was higher in Siha and Hela cell lines compared to that in the H8 cell line. The Siha cell line burdening Tim-4 lentiviral vector was successfully constructed. Over-expression of Tim-4 significantly inhibited the migration and invasion of cervical cancer cell line, and affected the expression of MMP2, MMP9, N-cadherin and E-cadherin. Conclusion：Over-expression of Tim-4 promotes the invasion and migration by regulating the EMT transformation in cervical cell carcinoma.

山东省自然科学基金资助项目（No.ZR2015HM031， ZR2014HM042）；山东省医药卫生科技发展计划(No. 2016WSA03022)