目的： 检测锌转运体1(zinc transporter 1，ZnT1)基因在胶质瘤组织中的表达，初步探索ZnT1对U87细胞增殖、迁移和 侵袭能力的影响。 方法： 收集2015年10月至2017年1月中国医科大学附属第一医院神经外科收治的术前未接受过放化疗的 Ⅱ～Ⅲ期胶质瘤患者20例，采用Real-time PCR和Western blotting检测胶质瘤组织与瘤旁组织中ZnT1 mRNA和蛋白的含量。向 胶质瘤细胞系U87中分别转染ZnT1和si-ZnT1质粒，构建ZnT1过表达和低表达细胞系，MTT和Transwell实验分别检测ZnT1对 U87细胞增殖、侵袭和迁移的影响。 结果： ZnT1 mRNA和蛋白在胶质瘤组织中表达显著高于瘤旁组织（均P<0.05）。成功构建 ZnT1过表达和低表达U87细胞系。与空白和空质粒对照组相比，转染12 h后，ZnT1过表达U87细胞的增殖（0.54±0.01 vs 0.45± 0.04、0.43±0.03，P<0.01）、侵袭和迁移能力（均P<0.05）显著升高；而转染12 h后ZnT1低表达U87细胞的增殖（0.37±0.03 vs 0.45±0.01、0.44±0.03，P<0.01）、侵袭和迁移能力（均P<0.05）显著降低。 结论： ZnT1在胶质瘤组织中呈高表达，ZnT1可以促进 胶质瘤U87细胞的增殖、迁移和侵袭。

Objective: To detect the expression of zinc transporter 1 (ZnT1) gene in glioma tissue, and to explore its effect on the prolif- eration, migration and invasion of U87 cells. Methods: From October 2015 to January 2017, 20 patients with glioma, who received no chemoradiotherapy before operation, were collected from Department of Neurosurgery, the First Affiliated Hospital of China Medical University. The protein and mRNA content of ZnT1 in glioma tissues and adjacent tissues were detected by Western blotting and Real- time PCR, respectively. ZnT1 and si-ZnT1 plasmids were transfected into glioma U87 cell line respectively to construct ZnT1 over-ex- pression U87 cell line and ZnT1 knockdown U87 cell line. The effects of ZnT1 on proliferation, migration and invasion of U87 cells were detected by MTT and transwell assay. Results: Both mRNA and protein expressions of ZnT1 in glioma tissues was significantly higher than those in adjacent tissues (all P<0.05). U87 cell lines with ZnT1 over-expression and knockdown were successfully construct- ed. Compared with the control group and empty plasmid control group, the proliferation (0.54±0.01 vs 0.45±0.04, 0.43±0.03, P<0.01), invasion and migration (all P<0.05) of U87 cells with ZnT1 over-expression were significantly increased at 12 h after transfection; how- ever, the proliferation (0.37±0.03 vs 0.45±0.01, 0.44±0.03, P<0.01), invasion and migration (all P<0.05) of U87 cells with ZnT1 knock- down were decreased significantly. Conclusion: ZnT1 was highly expressed in glioma tissues, and promoted the proliferation, migra- tion and invasion of glioma U87 cells.

教育部留学回国人员科研启动基金资助项目（教外司留[2013]1792号）；辽宁省自然科学基金面上项目资助（No.2015020460）；中国 博士后科学基金第59批面上项目资助（No.2016M590240）；沈阳市科技计划项目资助（No.17-230-9-13）；中国医科大学附属第一医院科学研究基 金资助(No.FSFH201722)