目的： 检测转录因子叉头框（forkhead box, FOX)基因家族成员FOXD1在不同级别胶质瘤组织中的表达，分析FOXD1 表达高低与胶质瘤患者预后之间的关系。 方法： 收集40例中国医科大学附属第一医院神经外科2014年9月至2015年2月行手 术治疗的胶质瘤患者肿瘤组织标本，7例颅脑外伤患者内减压切除组织作为对照。通过qRT-PCR以及免疫组化方法分析FOXD1 在胶质瘤与正常脑组织中的表达，并行临床病理因素相关性分析。采用Kaplan-Meier法分析FOXD1表达与胶质瘤患者生存期之 间的关系，通过检索GEO(GSE4290、GSE2223)及Rembrandt等基因数据库验证FOXD1在胶质瘤组织的表达及其与患者预后的关 系。 结果： qRT-PCR结果显示WHO Ⅳ级胶质瘤FOXD1的相对表达量高于正常脑组织以及II级胶质瘤组织（均P<0.01），免疫组 化结果显示在胶质瘤组织中FOXD1蛋白的表达与患者WHO病理级别(χ 2 =11.73，P<0.01)关联；FOXD1高表达组和低表达组生存 率之间差异具有统计学意义（P=0.043）。多个基因数据库检索资料验证结果证实，FOXD1 mRNA在胶质瘤中的表达高于正常脑 组织，FOXD1 mRNA表达的升高与胶质瘤患者术后生存期呈负相关。 结论 ：FOXD1在胶质瘤组织中高表达，并且随肿瘤级别升 高表达增高，FOXD1的高表达与胶质瘤患者预后呈负相关。

Objective: To evaluate the expression level of FOXD1 in glioma tissues of different grades, and to investigate the correla- tion between the expression of FOXD1 and the prognosis of glioma patients. Methods: The tumor tissues were collected from 40 glio- ma patients, who received surgical treatment in the neurosurgery department of the First Hospital of China Medical University from September 2014 to February 2015; Seven non-tumor tissues obtained from patients underwent internal decompression for traumatic brain injury were used as controls. The FOXD1 expression in glioma and non-tumor brain tissues was analyzed by qRT-PCR and IHC, and the correlations between clinical pathological features of glioma patients and FOXD1 expression level were analyzed. Furthermore, the Kaplan-Meier method was used to analyze the relationship between FOXD1 expression and survival time of patients. In addition, the expression of FOXD1 in glioma tissues and its relationship with patients’prognosis were confirmed by the data from GEO (GSE4290, GSE2223) and Rembrandt database. Results: qRT-PCR showed that the FOXD1 mRNA expression in glioma tissues of WHO grade IV was significantly higher than that of non-tumor brain tissues and glioma tissues of WHO grade II (P<0.01). German im- munohistochemical score (GIS) was used to evaluate the immunohistochemical staining intensity, and the relationship between FOXD1 expression and clinical pathological features was analyzed. The results showed that FOXD1 in glioma tissues was related to WHO pha- thological grade level (χ 2 =11.73，P<0.01). There was statistically significant difference between the survival time of FOXD1 high ex- pression group and FOXD1 low expression group (P=0.043). The data from GEO data base (GSE4290, GSE2223) and Rembrandt data- sets showed that glioma tissues have a higher FOXD1 mRNA expression level than normal brain tissues, and the elevated expression of FOXD1 mRNA was negatively associated with the survival time of glioma patients. Conclusion: FOXD1 was highly expressed in glio- ma tissues, and the expression level of FOXD1 was increased as the pathological grade increases. The elevated expression of FOXD1 was related with the poor survival of glioma patients.

辽宁省科学技术计划资助项目(No.2012225014)