目的： 制备负载光敏剂二氢卟吩e6 （chlorin e6，Ce6）的金纳米星（gold nanostars，GNS），研究其对肺癌A549细胞的光 动力作用效果。 方法： GNS经巯基聚乙二醇（SH-PEG-NH 2 )修饰后与光敏剂Ce6振荡过夜，制备出具有光动力治疗效应的探针 GNS-PEG@Ce6，检测其表征、形态和包封率，通过激光共聚焦显微镜比较A549细胞对探针GNS-PEG@Ce6与Ce6的吞噬作用。 应用MTT法检测探针GNS-PEG@Ce6对A549细胞增殖的抑制效果，通过流式细胞仪检测探针GNS-PEG@Ce6与Ce6对A549细 胞凋亡的影响。 结果： 探针GNS-PEG@Ce6的粒径为100 nm左右，具有良好的分散性和稳定性，Ce6的包封率为50%左右。探 针GNS-PEG@Ce6以胞吞方式进入细胞，主要分布于细胞质内，且较Ce6能更有效地进入细胞。探针GNS-PEG@Ce6对A549细 胞的增殖抑制作用强于Ce6 （P<0.05），流式细胞仪检测证明了探针对细胞有极为明显的致凋亡效果。 结论： 以GNS作为载体能 有效地增加肺癌A549细胞对Ce6的摄取，从而进一步增强Ce6对肺癌A549细胞的杀伤效果。

Objective: To prepare GNS (gold nanostars) loading photosensitizer chlorin e6 (Ce6) and to investigate its photodynamic effects on lung cancer A549 cells. Methods: GNS was firstly modified by SH-PEG-NH 2 and then mixed with Ce6 and shaken overnight to prepare GNS-PEG@Ce6, which had photodynamic therapy effects. The characterization, morphology and encapsulation rate were de- tected. The difference between the phagocytosis of Ce6 and GNS-PEG@Ce6 by A549 cells were observed with a Leical TCS SP8 con- focal laser scanning microscope. MTT assay was used to examine the inhibitory effect of GNS-PEG@Ce6 on the proliferation of A549 cells while FCM was used to detect the effect of probe GNS-PEG@Ce6 on the apoptosis of A549 cells. Results: The particle size of the GNS-PEG@Ce6 was about 100 nm. The prepared GNS-PEG@Ce6 nanoparticles exhibited good dispersion and stability and the encap- sulation rate of Ce6 was about 50%. GNS-PEG@Ce6 entered the cells by endocytosis and mainly distributed in the cytoplasm; com- pared with Ce6, GNS-PEG@Ce6 could enter the cells more effectively. The proliferation-suppression effect of GNS-PEG@Ce6 on A549 cells was significantly stronger than that of Ce6 (P<0.05). The results of flow cytometry showed that the probe exhibited strong apoptotic effect on A549 cells. Conclusion: GNS, as the drug carrier, could effectively increase the Ce6 uptake efficacy in A549 cells, thus further enhancing the killing effects of Ce6 on lung cancerA549 cells.

国家重点研发计划资助项目（No. 2017YFA0205301，No. 2015CB931802）；上海市经信委资助项目(No. XC-ZXSJ-02-2016-05)