[摘要] 目的：探讨半乳糖凝集素3（galectin-3）在膀胱癌组织中的表达与患者临床病理特征的关系及其对膀胱癌T24 细胞增殖、侵袭和凋亡的影响。方法：收集2014 年5 月至2016 年6 月在川北医学院附属医院收治的、经病理切片确诊的104 例膀胱癌患者的癌和癌旁组织标本，用免疫组织化学法检测膀胱癌和癌旁组织中半乳糖凝集素3 蛋白的表达，分析其表达与患者临床病理特征的相关性。将siRNA-Gal3、siRNA-Control 分别转染至T24 细胞，用Weatern blotting 检测细胞的半乳糖凝集素3 蛋白表达水平，MTT法检测细胞的增殖，Transwell 实验检测细胞的侵袭，流式细胞术检测细胞的凋亡。结果：膀胱癌组织中半乳糖凝集素3 阳性表达率显著高于癌旁组织（73.1% vs 9.6%，P<0.05），膀胱癌组织中半乳糖凝集素3 的表达与组织学分级、肿瘤的浸润深度、淋巴结转移和TNM分期均有关（均P<0.05），与性别和年龄无关（P>0.05）。siRNA-Gal3 显著下调半乳糖凝集素3 蛋白的表达水平（P<0.05），干扰半乳糖凝集素3 表达后：T24 细胞的增殖能力和侵袭能力均明显降低（均P<0.05）；细胞的凋亡率明显增加（P<0.05）。结论：半乳糖凝集素3 在膀胱癌组织中呈高表达，并与膀胱癌患者临床病理特征存在密切关系。干扰半乳糖凝集素3 蛋白的表达可抑制膀胱癌T24 细胞的增殖与侵袭，促进细胞的凋亡。

[Abstract] Objective: To investigate the expression of galectin-3 in bladder cancer tissues and its correlation with clinicopathological characteristics of bladder cancer patients, as well as to explore its effect on the proliferation, invasion and apoptosis of bladder cancer T24 cells. Methods: A total of 104 cases of pathologically confirmed bladder cancer tissues and the corresponding adjacent tissues were collected from the patients treated at the Affiliated Hospital of North Sichuan Medical College from May 2014 to June 2016. Immunohistochemistry staining was used to determine the galectin-3 protein expression in both cancer and adjacent tissues, and the correlations between galectin-3 expression and clinical pathological features were analyzed. siRNA-Gal3 and siRNA-Control were transfected into T24 cell, respectively.The expression of galectin-3 protein was detected by Western blotting, the proliferation of cells was detected by MTT assay; the invasion of cells was detected by Transwell assay; and the cell apoptosis was determined by Flow cytometry. Results:The positive rate of galectin-3 in bladder cancer tissues was significantly higher than that in adjacent tissues (73.1% vs 9.6%, P<0.05).The expression of galectin-3 in bladder cancer was correlated with histological grade, depth of invasion, lymph node metastasis and TNM stage (all P <0.05), but not with sex and age (P>0.05). The expression of galectin-3 was down-regulated significantly by siRNAGal3 (P<0.05). After interference with galectin-3, the proliferation and invasion of T24 cells was significantly decreased (all P<0.05)but the apoptosis was significantly increased (P<0.05). Conclusion: Galectin-3 is over-expressed in bladder cancer and is closely relat-ed to the clinicopathological features of bladder cancer patients. Interference of galectin-3 protein expression can inhibit proliferation and invasion and promote cell apoptosis of bladder cancer T24 cells.

中央财政支持地方高校发展专项资金(No. 2011JTD0030)