[摘要] 目的: 探讨shRNA 干扰骨形成蛋白和激活素的穿膜抑制剂(bone orphogenetic protein and activin membrane bound inhibitor, BAMBI)基因对人结肠癌SW480 细胞增殖、凋亡、侵袭和迁移的影响及其作用机制。方法: 转染SW480 细胞sh-BAMBI成功后，实时定量PCR（qRT-PCR)和Western blotting 检测BAMBI mRNA和蛋白表达水平，MTT法检测SW480 细胞增殖能力，Hoechst33258 染色检测细胞凋亡情况，Transwell 实验检测细胞侵袭能力，划痕实验检测SW480 细胞迁移能力，Western blotting检测TGF-β/Smad2 通路相关蛋白的表达水平。结果: 转染成功后sh-BAMBI组中BAMBI的mRNA和蛋白水平均低于对照组(P<0.05)；与对照组相比，sh-BAMBI 组细胞增殖率明显降低(P<0.05)、细胞凋亡率显著升高(P<0.01)，同时其侵袭和迁移能力明显减弱(均P<0.05)。sh-BAMBI组TGF-β 蛋白水平和p-Smad2/Smad2 比值明显高于对照组(P<0.05)。结论: shRNA干扰BAMBI可诱导人结肠癌SW480 细胞凋亡并抑制细胞增殖、侵袭和迁移，其机制可能与激活TGFβ/Smad2 通路有关。

[Abstract] Objective: To explore the effect of shRNA interfering BAMBI (bone morphogenetic protein and activin membrane bound inhibitor) on the proliferation, apoptosis, invasion and migration of human colon cancer SW480 cells and the possible mechanisms.Methods: After successful transfection with sh-BAMBI in SW480 cells, the mRNA and protein epxressions of BAMBI were detected by qRT-PCR and Western blotting, respectively. Cell proliferation was measured by MTT; apoptosis was tested by Hoechst33258 staining;cell invasion was detected by transwell assay; and cell migration was measured by wound healing assay. The expressions of TGF-β/Smad/2 signaling pathway related proteins were detected by Western blotting. Results: The mRNA and protein levels of BAMBI in sh-BAMBI group were lower than those of control group (P<0.05). Compared with control group, cell proliferation in sh-BAMBI group was obviously decreased (P<0.05), while apoptosis was obviously increased (P<0.01); in the meanwhile, cell invasion and migration in sh-BAMBI group were significantly reduced (P<0.05). In addition, the protein level of TGF-β and the ratio of p-Smad/2/ Smad/2 in sh-BAMBI group were significantly higher than those in control group (P<0.05). Conclusion: Interference of BAMBI by shRNA inhibits proliferation, invasion and migration but induces apoptosis of human colon cancer SW480 cells and activates TGF-β/Smad/2 pathway.

重庆市科技惠民计划资助项目（No.cstc2015jcsf10007)