目的：研究微小RNA-1180-5p（miR-1180-5p）对前列腺癌细胞株VCAP和LNCaP恶性生物行为的影响及可能的作用机制。方法：合成dsControl （dsControl 组）和miR-1180-5p（miR-1180-5p 组），分别转染至两个前列腺癌细胞株VCAP和LNCaP。采用qPCR 和Western blotting 分析转染后各组细胞CDKN1A、Cyclin D1 和CDK6 mRNA及蛋白的表达变化，采用流式细胞术、MTT法、平板克隆实验和Transwell 实验分别检测细胞周期分布、增殖活力、克隆形成能力、细胞迁移和侵袭能力。结果：qPCR结果显示，相比dsControl，转染miR-1180-5p 后VCAP 和LNCaP 细胞中CDKN1A mRNA 表达明显上调(P<0.01)；Cyclin D1 和CDK6 mRNA表达明显下调(P<0.05 或P<0.01)。Western blotting 与qPCR 结果相符。转染miR-1180-5p 后VCAP和LNCaP 位于G0/G1 期的细胞比例增加(P<0.01)，而位于S 期和G2/M期的细胞比例减少(P<0.05)，细胞周期被阻滞在G0/G1 期。转染miR-1180-5p 后，两种前列腺癌细胞增殖活力较dsControl 组明显降低(P<0.05)，miR-1180-5p 组两种细胞的克隆数量明显较少（P<0.01），同时miR-1180-5p 组两组细胞迁移和侵袭能力均下降（P<0.01）。结论：miR-1180-5p 能显著激活前列腺癌细胞中CDKN1A基因的表达，从而抑制前列腺癌细胞的增殖、迁移和侵袭等恶性生物行为。

Objective: To study the effects of microRNA-1180-5p (miR-1180-5p) on malignant biological behaviors of prostate cancer VCAP and LNCaP cells and the possible mechanisms. Methods: dsControl (dsControl group) and miR-1180-5p (miR-1180-5p group)were constructed and then transfected into two prostate cancer cell lines VCAP and LNCaP. qPCR andWestern blotting were used to analyze the changes in mRNA and protein expressions of CDKN1A, Cyclin D1 and CDK6 after transfection. Cell cycle distribution, proliferation activity, clone formation capacity, cell migration and invasion ability were detected by flow cytometry, MTT assay, colony culture assay and Transwell assay, respectively. Results: qPCR results showed that compared with dsControl, CDKN1A mRNA levels in VCAP and LNCaP cells transfected with miR-1180-5p were up-regulated significantly, while the mRNA expressions of Cyclin D1 and CDK6 were significantly down-regulated (all P<0.01). Western blotting result was consistent with that of qPCR. The percentage of cells in G0/G1 phase was increased after transfection of miR-1180-5p (P<0.05), but the proportion of cells in S phase and G2/M phase was decreased and the cell cycle was arrested at G0/G1 phase (P<0.05). The proliferation activity of the two prostate cancer cells was significantly lower than that of the dsControl group after miR-1180-5p transfection (P<0.05), and the number of colonies in the miR-1180-5p group was significantly lower than that in the dsControl group (P<0.01). In the meanwhile, the cell migration and invasion ability in miR-1180-5p group was decreased (P<0.01). Conclusion: miR-1180-5p can significantly activate CDKN1A gene expression in prostate cancer cells and further inhibit the proliferation, migration and invasion of prostate cancer cells.

湖北省自然科学基金资助项目（No.2017CFB176）