目的：探讨环状RNA（circRNA) ciRS-7 在食管鳞状细胞癌（esophageal squamous cell carcinoma，ESCC）组织中的表达及对ESCC细胞株增殖、迁移和侵袭的影响。方法：选取河北医科大学第四医院2016 年5 月至2017 年4 月收治的60 例食管癌患者病理组织标本及配对癌旁组织，通过qRT-PCR 检测ciRS-7 的表达水平。过表达或者敲低ciRS-7 后，采用CCK-8 法检测ESCC细胞株TE1 的增殖情况，同时采用划痕实验和Transwell 实验分别检测细胞迁移和侵袭能力的变化。最后通过动物实验在体内进行验证。结果：ciRS-7 在ESCC组织中高表达，且表达水平与病理分级和淋巴结转移有关（均P<0.05）。过表达ciRS-7 后，ESCC细胞株TE1 的增殖、迁移和侵袭能力显著升高（均P<0.05）；沉默ciRS-7 后，TE1 细胞的增殖、迁移和侵袭能力均显著降低（均P<0.05）。动物实验结果显示，转染ciRS-7 表达质粒组裸鼠的肿瘤体积和质量均明显高于空载体组（均P<0.05）。免疫组化检测结果表明，转染ciRS-7 表达质粒组裸鼠的肿瘤组织中增殖相关抗原(ki67、PCNA)、转移相关抗原(MMP2、MMP9)表达明显高于空载体组（均P<0.05）。结论：ciRS-7 在食管癌组织中表达上调，并且会增强食管癌细胞的增殖、迁移和侵袭能力，提示ciRS-7 可以作为诊断和治疗食管鳞状细胞癌的潜在作用靶点。

Objective: To investigate the expression of ciRS-7 in esophageal squamous cell carcinoma (ESCC) and its effect on the cellular proliferation, migration and invasion. Methods: The cancer tissues and paired adjacent normal tissues from 60 ESCC patients treated in the Fourth Hospital of Hebei Medical University between May, 2016 and April, 2017 were selected for this study. The expressions of ciRS-7 were detected by qRT-PCR. After over-expressing or silencing of ciRS-7, the proliferation of ESCC cell line TE1 was measured by CCK-8 assay; and the migration and invasion were tested by wound healing assay and Transwell invasion assay，respectively.Finally, the effect was validated via animal experiment. Results: CiRS-7 was highly expressed in ESCC tissues (P<0.05), and its expression level was closely related to pathological grade and lymph node metastasis (P<0.05). Over-expression of ciRS-7 significantly increased the proliferation, migration and invasion (all P<0.05) of TE1 cells; while silencing of ciRS-7 remarkably suppressed the proliferation,migration and invasion (all P<0.05). Conclusion: CiRS-7 was up-regulated in ESCC and could enhance ESCC cell proliferation,migration and invasion, suggesting that ciRS-7 could be used as a potential target for the diagnosis and treatment of ESCC.

河北省自然科学基金资助项目（No.2016206410）