目的：探讨黑色素瘤相关抗原A家族（melanoma antigen A，MAGE-As）在食管癌患者外周血中的表达，分析其与食管癌患者临床病理学指标及预后的关系。方法：采用多重巢式PCR（multiplex semi-nested PCR）法检测153 例食管癌患者和30 例健康人外周血中MAGE-As mRNA 表达水平，并利用酶切的方法鉴定该家族成员MAGE-A1、A2、A3、A4和A6 的表达情况。结果：MAGE-As 在食管癌患者外周血中阳性表达率为19.61%（30/153），MAGE-A1、A2、A3、A4、A6 的阳性表达率分别为10.46%（16/153）、16.34%（25/153）、9.8%（15/153）、11.11%（17/153）和18.30%（28/153）。MAGE-As 阳性表达与临床分期、远处转移、淋巴结转移呈正相关（均P<0.05），MAGE-As 及其亚型基因的阳性表达均与食管癌患者5 年生存率较差相关（均P<0.05）；MAGE-As 表达、肿瘤大小、淋巴结转移及远处转移可作为食管癌患者生存的独立预后因素（均P<0.01）。结论：MAGE-As在食管癌患者外周血中的表达与食管癌预后相关，有可能作为监测食管癌预后的重要指标。

Objective: To evaluate the expression of melanoma antigen A family（MAGE-As）in the peripheral blood of patients with esophageal carcinoma (EC), and to analyze its correlations to the clinicopathological features and the prognosis of EC patients. Methods:mRNA expression of MAGE-As in peripheral blood from 153 EC patients and 30 healthy donors was detected using multiplex semi-nested PCR. In addition, restriction endonuclease treatment was used to determine the expression of MAGE-As family members,including MAGE-A1, A2, A3, A4 and A6. Results: The positive expression of MAGE -As was observed in 30 of 153 EC patients (19.61%) in peripheral blood. The positive expression rate of MAGE-A1, A2, A3, A4, A6 was 10.46% (16/153), 16.34%(25/153), 9.8% (15/153), 11.11% (17/153) and 18.30%(28/153), respectively. Additionally, the expression of MAGE-As was positively associated with clinical stage, lymphatic metastasis and distant metastasis (all P<0.05). The positive expressions of MAGE-As and its sub-type genes were all associated with low 5-year overall survival of ES patients (all P<0.05). Expression of MAGE-As, tumor volume, lymphatic metastasis and distant metastasis can be used as independent prognostic factors for the survival of EC patients (all P<0.01). Conclusion:The expression of MAGE-As in peripheral blood of EC patients was associated with the prognosis of EC, and may be used as an important indicator for the prognosis of esophageal carcinoma.

河北省财政支撑项目资助（No.[2014]1257）；河北省杰出青年基金资助项目（No.H2016206410）；河北省财政厅资助项目（冀财No.[2016]361006