目的: 评价脐带血来源单个核细胞（umbilical cord blood mononuclear cell ，UCMC)诱导分化的免疫细胞对小细胞肺癌（small cell lung cancer ，SCLC）患者免疫功能的影响。方法：选取2012 年1 月至2015 年12 月就诊于内蒙古医科大学附属医院的90 例SCLC 患者，采用分层随机方法分为对照组（45 例，EP 方案）和研究组（45 例，EP 方案+UCMC诱导分化的免疫细胞），研究组患者在化疗结束后3～5 d 进行一个疗程的UCMC 诱导分化的免疫细胞治疗[（1~3）×1010 个细胞/疗程] ，每30 d 为一个周期。治疗前及治疗结束后12 周，采用流式细胞术检测患者外周血中T 细胞亚群的变化及相关因子IFN- γ、IL-2、IL-10、TGF- β1 的变化，评价患者生活质量和不良反应等。结果：研究组获得CR 15 例、PR 25 例、SD 5 例，研究组T 细胞亚群含量较对照组有明显增加（P<0.01）、并且恢复正常范围的时间明显短于对照组（P<0.05）；80.9% 的患者炎性细胞因子IFN- γ 在血清中升至或高于正常范围，IL-10 、TGF- β 1 较治疗前降低（P<0.05 ）；患者生活质量较对照组有显著提高。结论：UCMC 诱导分化的免疫细胞可以促进SCLC 患者化疗后免疫功能的恢复。

Objective: To evaluate the effect of immune cells induced and differentiated by umbilical cord blood mononuclear cells (UCMCs) on the immune function of patients with small cell lung cancer (SCLC). Methods: Ninety patients with SCLC, who were admitted to the Affiliated Hospital of Inner Mongolia Medical University from January 2012 to December 2015, were randomly divided into control group (45 patients, EP regimen), study group (45 patients, EP regimen+UCMC-induced and differentiated immune cells). The study group of patients received immune cell treatment 3-5 d after chemotherapy ([1-3]×1010cells/treatment), 30 d for a cycle. The changes in T cell subsets, IFN- γ , IL-2, IL-10 and TGF- β1 in peripheral blood of patients were observed by flow cytometry at pre-treatment and 12 weeks post-treatment. Life quality and adverse events of patients were evaluated. Results: The study group, 15 cases achieved CR, 25 cases of PR and 5 cases of SD. The percent of T cell subsets in the study group was significantly higher than that in the control group (P<0.01), and the time of return to normal level was obviously shorter (P<0.05). The serum level of inflammatory cytokine IFN- γ increased or exceeded the normal range in 80.9% patients, and IL-10 and TGF- β1 levels were significantly decreased as compared with pretreatment (P<0.05). The quality of life was obviously better than that of the control group (P<0.05). Conclusion: Immune cells induced and differentiated by UCMCs can promote the recovery of immune function of patients with SCLC.

国家自然科学基金资助项目（No. 81360354）；内蒙古自治区科技计划资助项目（No. 20130401）