目的：探讨hsa-miR-150-5p 靶向低氧诱导因子1α（hypoxia inducibility factor 1，HIF1α）对成胶质细胞瘤(glioblastoma，GBM)U-251MG细胞恶性生物学行为的影响及其作用机制。方法：qRT-PCR检测miR-150-5p 及其HIF1α mRNA在U-251MG细胞中的表达水平，荧光素酶报告实验验证miR-150-5p 和HIF1α 之间的生物学关系及miR-150-5p 和HIF1α 在U-251MG细胞中的生物学功能，Western blotting 检测miR-150-5p 及HIF1α 蛋白在U-251MG细胞中的表达,Transwell 实验检测U-251MG细胞的侵袭能力,划痕实验检测U-251MG细胞的迁移能力。结果：miR-150 mimic 转染U-251MG细胞后，HIF1α mRNA表达水平明显下调（P<0.01），HIF1α 蛋白水平也明显下调（P<0.01)。miR-150-5p 降低了wt HIF1α 3’-UTR转染细胞的荧光素酶活性（P<0.05），证实miR-150-5p 通过结合HIF1α 的3'-UTR负调控HIF1α 的表达。在U-251MG细胞中，miR-150-5p 过表达可明显抑制HIF1α 蛋白表达、细胞侵袭和迁移（均P<0.05）。结论：miR-150-5p 通过负调控HIF1α 抑制U-251MG细胞的侵袭和转移，表明miR-150-5p 和HIF1α 有可能是GBM潜在的治疗靶点。

Objective: To explore the effect and possible mechanisms of has-miR-150-5p targeting HIF1α to regulate malignant biological behaviors of glioblastoma (GBM) U-251MG cells. Methods: Real-time quantitative PCR (RT-PCR) was used to detect the expression of miR-150-5p and hypoxia inducible factor 1 (HIF1α) in U-251MG cells. Luciferase report assay was carried out to verify the biological relationship between miR-150-5p and HIF1α and their biological functions in U-251MG cells. The protein expressions of miR-150-5pand HIF1α in U-251MG cells were detected by western blotting. The ability of cell migration was detected by wound healing test and cell invasion ability was detected by transwell test. Results: After miR-150-5p mimic transfection, the mRNA expression of HIF1α was significantly reduced in U-251MG cells (P<0.01). Bioinformatics prediction and luciferase reporter assay demonstrated that miR-150-5p down-regulated HIF1α through directly binding to HIF1α 3’- untranslated region (3’-UTR) (all P<0.05). In U-251MG cells, miR-150-5p over-expression significantly inhibited HIF1α expression, cell invasion and migration (all P<0.05). Conclusion: miR-150-5p inhibits cell invasion and metastasis through negative regulation of HIF1α, indicating that miR-150-5p and HIF1α were both potential therapeutic targets for glioblastoma.

四川省卫生和计划生育委员会资助课题（No.1504122）