目的:检测成蛋白2（formin-2，FMN2）在胃腺癌组织中的表达水平，探讨FMN2 的表达与胃癌患者临床特征之间的关系及其对胃腺癌AGS细胞增殖的影响。方法:收集2015 年9 月至2017 年9 月空军军医大学第一附属医院消化病医院胃肠外科手术切除的胃腺癌组织及相应的癌旁组织标本84 例。采用免疫组织化学染色和肿瘤数据库检索检测分析胃腺癌组织中FMN2的表达水平，并探讨FMN2 蛋白表达与胃腺癌患者临床病理特征之间的相关性；MTT法检测FMN2 对胃腺癌AGS细胞增殖活性的影响，Western blotting 法检测FMN2 对胃腺癌AGS细胞凋亡蛋白Caspase-3 表达的影响。结果:胃腺癌组织中FMN2 表达水平明显低于癌旁组织（P<0.05 或P<0.05)，其低表达与胃腺癌患者的TNM分期及肿瘤分化程度相关（均P<0.05）；与AGS细胞对照比较，AGS/FMN2 稳转株的增殖率明显降低、凋亡相关蛋白Caspase-3 表达明显增加（均P<0.05）。结论:FMN2 在胃腺癌组织中表达明显下调，且与患者的肿瘤分化程度和TNM分期相关；过表达FMN2 的胃腺癌细胞其增殖率明显降低；FMN2 是胃癌预后的独立危险因素，可为胃腺癌的治疗提供新的思路。

Objective: To investigate the expression of Formin-2（FMN2）protein in gastric cancer tissues and its correlation to clinicopathological features of gastric cancer patients, as well to explore its effect on the proliferation of gastric adenocarcinoma AGS cells.Methods: 84 cases of gastric adenocarcinoma tissues and corresponding para-cancerous tissues were surgically collected from patients treated in the First Affiliated Hospital of Air Force Military Medical University from September 2015 to September 2017. The expression of FMN2 in gastric adenocarcinoma tissues was detected by immunohistochemical staining and analyzed with RNA-Seq data-sets GEPIA. The relationship between FMN2 protein expression in gastric adenocarcinoma tissues and its clinicopathological features was also explored. MTT assay was used to detect the effect of FMN2 on AGS cell proliferation activity, and Western blotting was used to detect the effect of FMN2 on the expression of apoptosis-related protein caspase-3 in AGS cells. Results: The expression level of FMN2 in gastric adenocarcinoma tissues was significantly lower than that in matched adjacent tissues and the expression level of FMN2 was closely related to the TNM stage and differentiation of gastric adenocarcinoma (all P<0.05). Compared to AGS control group, the proliferation activity of AGS/FMN2 was significantly decreased and the expression of apoptosis-related gene Caspase-3 was markedly increased (all P<0.05). Conclusion: The expression of FMN2 was significantly decreased in gastric adenocarcinoma tissues and its low expression is closely related to the degree of tumor differentiation and clinical TNM stage. Moreover, FMN2 over-expression significantly decreased the proliferation of AGS cells. FMN2 may function as independent risk factor for the prognosis of gastric adenocarcinoma,which may provide new ideas for the treatment of gastric adenocarcinoma.

陕西省社会发展与攻关基金资助项目（No. 2014SF2-10）