目的：探讨半乳凝集素-3（galectin-3）蛋白在人乳腺癌组织中的表达规律及沉默galectin-3 基因后对乳腺癌MCF-7 细胞迁移、侵袭和凋亡的影响。方法：收集2014 年2 月至2018 年2 月邢台市人民医院手术切除的15 例乳腺癌患者癌组织及其癌旁组织，另外采集该医院和河北医科大学附属第四医院组织石蜡切片100 份，利用Western blotting 检测15 例乳腺癌患者的癌组织及癌旁组织中galectin-3 蛋白相对表达水平，用免疫组织化学法检测galectin-3 蛋白在100 例乳腺癌石蜡标本切片中的表达水平，并分析其表达与患者临床病理特征的关系。将galectin-3 siRNA 转染至MCF-7 细胞中，用qPCR 法和Western blotting 分别检测galectin-3 mRNA和蛋白的表达水平；用Transwell 小室法和流式细胞术分别检测galectin-3 基因沉默后对MCF-7 细胞迁移、侵袭和凋亡的影响。结果：在乳腺癌组织中galectin-3 蛋白表达水平显著高于癌旁组织（P<0.05）；乳腺癌组织中galectin-3 蛋白阳性表达率为67.00%，其表达水平在淋巴结转移、激素受体（ER、PR）阴性组中显著升高（均P<0.05），且随TNM分期和组织学分级的升高而升高（均P<0.05）。转染galectin-3 siRNA 后，能显著降低MCF-7 细胞galectin-3 mRNA和蛋白的表达水平、迁移和侵袭能力（均P<0.05），提高细胞的凋亡率（P<0.05）。结论：Galectin-3 在乳腺癌组织中高表达，沉默galectin-3 表达后抑制MCF-7 细胞的迁移和侵袭、诱导细胞凋亡，可作为乳腺癌生物治疗的一个新靶点。

Objective: To investigate the expression of galectin-3 protein in human breast cancer tissues and the effect of silencing galectin-3 gene on the migration, invasion and apoptosis of human breast cancer MCF-7 cells. Methods: The relative expression of galectin-3 protein in 15 cases of breast cancer tissues and corresponding para-cancerous tissues were detected by Western blotting; The expression of galectin-3 protein in paraffin sections of 100 cases of breast cancer tissues were detected by immunohistochemistry, and the correlation between galectin-3 expression and the clinicopathological characteristics of breast cancer patients was also analyzed. Galectin-3 siRNA were transfected into human breast cancer MCF-7 cells by liposome, then Real-time PCR and Western blotting were used to detect the mRNA and protein expression of galectin-3. The effect of galectin-3 gene silencing on cell migration and invasion ability of MCF-7 cells were detected by Transwell method. The effect of galectin-3 gene silencing on apoptosis of MCF-7 cells were detected by flow cytometry. Results: Western blotting detection showed that the relative expression of galectin-3 protein in breast cancer tissues were significantly higher than that in para-cancerous tissues (P<0.05);Immunohistochemistry detection showed that the positive expression rate of galectin-3 protein in breast cancer tissues was 67.00%, the positive expression rates in the lymph node metastasis, hormone receptor (ER, PR) negative groups were significantly higher (P<0.05), and the positive expression rate of galectin-3 protein were increased with the increase of TNM stage and histological grade (P<0.05); Galectin-3 siRNA transfection could significantly reduce the mRNA and protein expression of galectin-3 in MCF-7 cells (P<0.05), and reduce the invasion and migration ability but significantly improve the rate of apoptosis of MCF-7 cells (P<0.05). Conclusion: Galectin-3 is highly expressed in breast cancer tissues, and its silence can inhibit the invasion and metastasis of MCF-7 cells and induce apoptosis of MCF-7 cells. Galectin-3 can be used as a new target for biological therapy of breast cancer.

河北省医药卫生科技发展支持项目（No. 2014JQE27031）