目的:探讨miR-138-5p 与T细胞因子3 基因（T cell factor 3, TCF3）的靶向关系及其对人胃癌细胞SGC-7901 侵袭和迁移能力的影响。方法：miR-138-5p 模拟物转染SGC-7901 细胞后，实时荧光定量PCR（qRT-PCR）检测miR-138-5p 和TCF3 mRNA的相对表达；生物信息学方法预测miR-138-5p 与TCF3 基因的靶向匹配关系，采用荧光素酶报告基因系统鉴定该关系。miR-138-5p 转染正常胃癌细胞和TCF3 高表达胃癌细胞后，Western blotting 检测TCF3、神经钙黏蛋白（N-cadherin）、波形蛋白（Vimentin）、锌指转录因子（Slug）和上皮型钙黏蛋白（E-cadherin）的表达，Transwell 小室检测胃癌细胞侵袭能力，划痕实验检测细胞迁移能力。结果：miR-138-5p 过表达抑制TCF3 mRNA的表达；生物信息学软件预测显示miR-138-5p 与TCF3 mRNA有靶向结合区域，miR-138-5p mimic 降低TCF3 野生型质粒的荧光素酶活性，不影响TCF3 突变型质粒的荧光素酶活性。miR-138-5p 抑制TCF3 蛋白表达，miR-138-5p 减弱TCF3 过表达对SGC-7901 细胞侵袭和迁移能力的促进作用，同时其下调N-cadherin、Vimentin 和Slug 蛋白表达、上调E-cadherin 蛋白表达。结论：miR-138-5p 抑制胃癌细胞SGC-7901 的侵袭和迁移，与直接靶向调控TCF3 的表达有关。

Objective: To explore the target relationship between miR-138-5p and TCF3, and its effect on invasion and migration of human grastric cancer SGC-7901 cells. Methods: After being transfected with miR-138-5p mimic, the relative mRNA expression of miR-138-5p and TCF3 in SGC-7901 cells was detected by qRT-PCR. Bioinformatics methods were used to predict the targeted matching relationship between miR-138-5p and TCF3 gene, which was then verified by the luciferase reporter gene system. After transfecting normal gastric cancer cells and TCF3 high expression gastric cancer cells with miR-138-5p mimic, Western blotting was performed to detect the protein expressions of TCF3, N-cadherin, Vimentin, Slug and E-cadherin; Transwell assay and scratch assay were used to detect the invasion and migration ability of gastric cancer cells, respectively. Results: Over-expression of miR-138-5p inhibited the expression of TCF3 mRNA. Bioinformatics software predicted that miR-138-5p and TCF3 mRNA had targeted binding areas.miR-138-5p mimics reduced the luciferase activity of TCF3 wild-type plasmids, without affecting the luciferase activity of TCF3 mutant-type plasmids. miR-138-5p inhibited the expression of TCF3 protein, and attenuated the promotion effect of TCF3 over-expression on the invasion and migration of SGC-7901 cells, in the meanwhile, down-regulated the protein expressions of N-cadherin, Vimentin and Slug proteins, and up-regulated the protein expression of E-cadherin. Conclusion: miR-138-5p directly regulates the expression of TCF3 and affects the invasion and migration of gastric cancer SGC-7901 cells.

河南省科技攻关计划资助项目（No. 162102310329）