目的:探讨miR-141-3p 与转化生长因子-β2(transforming growth factor β2, TGF-β2)基因的靶向关系及其对人前列腺癌细胞株C4-2B 恶性生物学行为的影响。方法：转染miR-141-3p mimic 后，qRT-PCR 检测C4-2B 细胞miR-141-3p 和TGF-β2mRNA的表达水平，双荧光素酶报告基因实验验证miR-141-3p 与TGF-β2 的靶向关系。miR-141-3p mimic 与TGF-β2 过表达载体单独或共转染C4-2B细胞后, Western blotting 检测转染C4-2B细胞中TGF-β2 蛋白的表达水平，Hoechst33258 染色检测细胞凋亡情况，Transwell 实验检测各组细胞的侵袭能力。结果：miR-141-3p mimic转染后，C4-2B细胞miR-141-3p 的相对表达水平明显提高、TGF-β2 mRNA的相对表达水平明显降低（均P<0.01）。miR-141-3p mimic 与野生型报告载体共转后，荧光素酶的活性显著降低（P<0.01）；miR-141-3p mimic 与突变型报告载体共转后，荧光素酶的活性没有明显变化（P>0.05）；miR-141-3p mimic 添加到pc-TGF-β2 转染的C4-2B细胞后，细胞增殖倍数明显降低、凋亡细胞数目显著增加、细胞侵袭能力显著降低（均P<0.01）。结论：miR-141-3p 与TGF-β2 存在靶向关系，且两者协同影响人前列腺癌C4-2B细胞增殖、侵袭能力并诱导其凋亡。

Objective: To investigate the relationship between miR-141-3p and transforming growth factorβ2 (TGF-β2), and its effects on the malignant biological behaviors of human prostate cancer cell line C4-2B. Methods: After the transfection of miR-141-3p mimic,the mRNA expression of miR-141-3p and TGF-β2 in C4-2B cells was detected by qRT-PCR. Bioinformatics method validated the relationship between miR-141-3p and TGF-β2. miR-141-3p mimic alone or with TGF-β2 over-expression vector was transfected into C4-2B cells, and then Western blotting was used to detect the expression of TGF- β2 protein in C4-2B cells, Hochest33258 staining was used to detect cell apoptosis, and Transwell assay was used to detect the invasion ability of cells in each group. Results: After the transfection of C4-2B cells with miR-141-3p mimic, the level of miR-141-3p increased significantly, and the level of TGF- β2 mRNA decreased significantly (all P<0.01). The activity of luciferase was significantly reduced after the co-transfection with miR-141-3p mimic and wild type report plasmid (P<0.01); However, the activity of luciferase was not obviously changed after co-transfection with miR-141-3p mimic and mutant type report plasmid (P>0.05). After co-transfection with miR-141-3p mimic and pc-TGF-β2, the proliferation of C4-2B cells decreased significantly, the number of apoptotic cells increased significantly, and the cell invasion ability decreased significantly (all P<0.01). Conclusion: miR-141-3p inhibits the proliferation and invasion of human prostate cancer C4-2B cells and induces cell apoptosis by targeting TGF-β2.

四川省科技厅资助项目（No. 2014JY0187）