目的:探讨丹参酮IIA 对食管癌EC9706 和KYSE70 细胞侵袭和迁移的影响及其调控机制。方法:食管癌细胞系EC9706 和KYSE70 培养完成后分为4 组：对照组（加DMSO）和2、4、6 μg/ml 丹参酮组。采用CCK-8 法检测EC9706 和KYSE70 细胞增殖活力，流式细胞术检测细胞凋亡率，Transwell 实验检测细胞侵袭能力，划痕愈合实验检测细胞迁移能力，qRT-PCR和Western blotting 实验检测EMT相关蛋白E-cadherin、Snail-2、Vimentin 和N-cadherin mRNA和蛋白表达水平。结果：小于6 μg/ml的丹参酮IIA 不影响食管癌EC9706 和KYSE70 细胞增殖活力；4、6 μg/ml 丹参酮IIA 组细胞凋亡率明显高于对照组（均P<0.01）；2、4、6 μg/ml 丹参酮组每个视野下的侵袭细胞数及划痕愈合率明显低于对照组（均P<0.01），且EC9706 和KYSE70 细胞形态由纺锤状的间充质形态转变为上皮形态。与对照组相比，2、4、6 μg/ml 丹参酮组E-cadherin 表达明显升高，Snail-2、Vimentin 和N-cadherin表达明显下降（均P<0.01）。结论：丹参酮IIA 通过抑制EMT促进食管癌EC9706 和KYSE70 细胞凋亡，并减弱其侵袭和迁移能力。

Objective: To investigate the effect of tanshinone IIA on the invasion and migration of esophageal cancer EC9706 and KYSE70 cells, and to explore the underlying mechanism. Methods: Esophageal cancer cells (EC9706 and KYSE70) were divided into 4 groups: control group, tanshinone IIA groups (2, 4, 6 μg/ml). Cell prliferation viability was measured by CCK-8; Apoptosis was detected by flow cytometry; Invasion was tested by Transwell assay; And migration was measured by Scratch assay. The mRNA and protein levels of E-cadherin, Snail-2, Vimentin and N-cadherin were tested by quantitative Real-time reverse transcription PCR (qRT-PCR)and Western blotting, respectively. Results: Tanshinone IIA at concentrations less than 6 μg/ml did not affect the cell viability of esophageal cancer EC9706 and KYSE70 cells. The apoptosis in tanshinone IIA (4, 6 μg/ml) groups was significantly higher than that in control group (P< 0.01). The number of invasive cells per field and wound-healing rate in tanshinone IIA (2, 4, 6 μg/ml) groups were significantly lower than those in control group (all P<0.01). Moreover, the cell morphology was transformed from a spindle-shaped mesenchymal form into epithelial morphology after tanshinone IIA treatment. Compared with control group, the expression of E-cadherin in tanshinone IIA groups (2, 4, 6 μg/ml) was significantly up-regulated while the expressions of Snail-2, Vimentin and N-cadherin were significantly down-regulated (all P<0.01). Conclusion: Tanshinone IIA promotes apoptosis and attenuates the invasion and migration of esophageal cancer cells by inhibiting the epithelial-mesenchymal transition.

四川省教育厅科研项目（No.18ZB0176）