[摘要] 目的:研究组织驻留CD8+T细胞（CD103+CD8+T细胞）在结直肠癌（colorectal cancer，CRC）组织中浸润程度及分布特征，分析其浸润程度与患者临床病理特征及预后的关系。方法:选用上海芯超生物科技有限公司的88 例结肠癌HColA180Su14和77 例直肠癌HRec-Ade180Sur-03 组织芯片，应用免疫荧光染色法分别检测CRC组织及相应癌旁组织中CD103+CD8+T细胞的浸润分布特征及程度，Wilcoxon 秩和检验比较CRC及癌旁组织中CD103+CD8+T细胞浸润程度，χ2检验分析CRC中CD103+CD8+T细胞浸润程度与患者临床病理特征的关系；Kaplan-Meier 生存分析CD103+CD8+T细胞浸润程度与患者预后的关系，拟合Cox 模型评价不同指标与患者预后的关系。结果: CRC组织中CD103+CD8+T 细胞浸润程度与癌旁组织比较差异无统计学意义（P>0.05），有远处转移患者中CD103+CD8+T细胞高度浸润的比率显著低于无远处转移患者（P<0.01），CD103+CD8+T细胞浸润程度与患者其他临床病理特征无明显相关（P>0.05）。Kaplan-Meier生存分析显示，CD103+CD8+T细胞高度浸润患者的OS较低度浸润患者显著延长（54.42% vs 25.00%，P<0.05），多因素Cox 显示，病理分级（P<0.01）和CD103+CD8+T细胞高度浸润（P<0.05）均可作为CRC患者预后的独立影响因素。结论: CRC组织中CD103+CD8+T细胞浸润与预后相关，提示其在CRC发生发展过程中发挥重要作用。

[Abstract] Objective: To investigate the degree and distribution of tissue-resident CD8+ T cell (CD103+CD8+T cells) infiltration in colorectal cancer (CRC) tissues, and to analyze its relationship to patients’clinicopathological features and prognosis. Methods: Tissue chips of 88 cases of colon cancer tissues (No.HColA180Su14) and 77 cases of rectal cancer tissues (No. HRec-Ade180Sur-03) were obtained from Shanghai Outdo Biotech Co.,Ltd. Immunofluorescence staining was performed to examine the infiltration pattern and degree of CD103+CD8+T cells in the collected CRC tissues and their para-cancerous tissues. Wilcoxon rank test was used to compare CD103+CD8+T cell infiltration degree in CRC tissues and the para-cancerous tissues. Chi-square test was used to analyze the relationship between CD103+CD8+T cell infiltration in CRC and patients’clinicopathological features. Kaplan-Meier survival analysis was conducted to explore the correlation between CD103+CD8+T cell infiltration and patients’prognosis. Cox model was applied to analyze the correlation between different clinical parameters and patients’prognosis. Results: CD103+CD8+T cell infiltration presented no signifi-cant differences between CRC and para-cancer tissues (P>0.05). Patients with distant metastasis had significantly lower CD103+CD8+T cell infiltration rate than patients without distant metastasis (P<0.01). There was no significant correlation between the infiltration of CD103+CD8+T cells and other clinicopathological features (P>0.05). Kaplan-Meier survival analysis showed that the overall survival (OS) of patients with high CD103+CD8+T cell infiltration was significantly longer than that of the patients with low infiltration (54.42%vs 25.00%, P<0.05). Multivariate Cox model analysis indicated that pathological grade (P<0.01) and high CD103+CD8+T cell infiltration (P<0.05) were independent prognostic factors for CRC. Conclusion：CD103+CD8+T cell infiltration in CRC is associated with patients’prognosis,suggesting that CD103+CD8+T cell plays an important role in the initiation and development of CRC.

国家重点研发资助项目（No. 2018YFC1313400）；国家科技支撑计划资助项目（No. 2015BAI12B12）；国家自然科学基金资助项目（No.31570877，No.31570908）；国家自然科学基金海外及港澳学者合作研究基金（No.31729001）；江苏省重点研发计划专项资金项目（No.BE2018645）