[摘要] 目的：探讨非受体型蛋白酪氨酸磷酸酶6（non-receptor protein tyrosine phosphatase 6，PTPN6）基因在不同食管鳞状细胞癌细胞株中的表达及其对Eca109、Yes-2 细胞恶性生物学行为的影响。方法：应用qPCR 法检测不同食管鳞癌细胞株（TE1、Eca109、Kyse150、Kyse170、Yes-2）中PTPN6 mRNA的表达水平，以pcDNA3.1-PTPN6 质粒分别瞬时转染食管鳞癌细胞株Eca109和Yes-2，应用qPCR和Wb法检测PTPN6 mRNA和蛋白的表达水平，并应用MTS、克隆形成实验、划痕实验和Transwell 法检测过表达PTPN6 基因对食管鳞癌细胞恶性生物学行为的影响。结果：PTPN6 基因在5 种食管鳞癌细胞侏中表达均明显下调（均P<0.05）。与转染空载体的对照组相比，经pcDNA3.1-PTPN6 转染后，Eca109 和Yes-2 细胞均高水平表达PTPN6（P<0.05 或P<0.01）；过表达PTPN6 基因后，Eca109 和Yes-2 细胞的增殖、迁移和侵袭能力均明显被抑制（均P<0.05）。结论：PTPN6 基因高表达能抑制食管鳞癌细胞的增殖、迁移和侵袭，其可能是影响食管鳞癌细胞生物学特性的一个重要因素。

[Abstract] Objective: To detect the expression of non-receptor protein tyrosine phosphatase 6 (PTPN6) in different esophageal squamous cell carcinoma (ESCC) cell lines, and to investigate its effect on proliferation, migration and invasion ability of Eca109 and Yes-2 cell lines. Methods: qPCR was applied to detect the mRNA expression of PTPN6 in different ESCC cell lines (TE1, Eca109, Kyse150,Kyse170 and Yes-2). pcDNA3.1-PTPN6 plasmid was transiently transfected into Eca109 and Yes-2 cells respectively. The expression of PTPN6 was detected by real-time PCR and Wb. The effects of PTPN6 over-expression on the biological behaviors of ESCC cells were detected by MTS, colony formation assay, wound healing assay and Transwell assay, respectively. Results: The mRNA expression of PTPN6 was remarkably reduced in ESCC cell lines (TE1, Eca109, Kyse150, Kyse170 and Yes-2) compared to normal esophageal epithelial cells (HEEpiC) (P<0.05). Compared to the mock cells, significant up-regulation of PTPN6 was detected in pcDNA3.1-PTPN6 transfected Eca109 and Yes-2 cells (all P<0.05 or P<0.01); PTPN6 over-expression led to a significant inhibition in migration and invasion ability of Eca109 and Yes-2 cells (all P<0.05). Conclusions: Over-expression of PTPN6 may inhibit the proliferation, migration and invasion of ESCC cells, which might be an important factor influencing the biological characteristics of ESCC cells.

国家自然科学基金资助项目（No.81572441）；河北省医学科学研究重点课题资助项目（No.20170700）