[摘要] 目的: 探讨miR-137 在宫颈癌组织和细胞中的表达及其对宫颈癌细胞增殖、迁移及侵袭的作用及其机制。方法: 选用2017 年1 月至2018 年3 月东莞市人民医院妇产科32 例手术切除的宫颈癌组织及对应的癌旁组织标本，以及宫颈癌细胞系C33A、HeLa、SiHa 及宫颈上皮永生化细胞株H8，用RT-PCR 法检测宫颈癌组织和细胞系中miR-137 的表达水平。将miR-137mimics、miR-137 NC质粒转染到C33A和HeLa 细胞，用CCK-8 法、Transwell 迁移及侵袭实验观察上调miR-137 表达对C33A和HeLa 细胞增殖、迁移和侵袭能力的影响。用荧光素酶报告基因及WB实验检测miR-137 和Wnt5a 在宫颈癌中的靶向调控关系。构建Wnt5a 过表达载体，观察同时过表达Wnt5a 和miR-137 对宫颈癌细胞系C33A和HeLa增殖、迁移和侵袭的影响。结果: 在宫颈癌组织和细胞系中miR-137 表达水平明显低于癌旁组织和H8 细胞（均P<0.05）。上调miR-137 表达能够显著抑制C33A和HeLa 细胞的增殖、迁移和侵袭能力（均P<0.05）。萤光素酶报告基因实验确定Wnt5a 与miR-137 的靶向关系。过表达Wnt5a 后，可以在一定程度上逆转miR-137 对宫颈癌细胞系C33A和HeLa 的增殖、迁移和侵袭能力。结论: miR-137 通过靶向Wnt5a 抑制宫颈癌细胞的增殖、迁移、侵袭，其可能是宫颈癌治疗的潜在靶点。

[Abstract] Objective：To investigate the expression of miR-137 in cervical cancer tissues and cells, and to explore its effect on proliferation,migration and invasion of cervical cancer cells as well as the mechanisms. Methods：Thirty-two pairs of cervical cancer tissues and corresponding para-cancerous tissues that surgically resected at the Department of Gynecology and Obstetrics of Dongguan People's Hospital from January 2017 to March 2018 were collected for this study. In addition, cervical cancer cell lines C33A, HeLa, SiHa and cervical epithelial immortalized cell line H8 were also collected. The expression of miR-137 in cervical cancer tissues and cell lines was detected by RT-PCR. miR-137 mimics and miR-137 NC were respectively transfected into C33A and HeLa cells, and the effects of miR-137 over-expression on proliferation, migration and invasion of cervical cancer cell lines were observed by CCK-8 and Transwell assay. Luciferase reporter gene assay and WB were used to determine the relationship between miR-137 and Wnt5a in cervical cancer.Wnt5a over-expression vector was constructed, and the effects of simultaneous over-expression of Wnt5a and miR-137 on proliferation,migration and invasion of C33A and HeLa cells were observed. Results：The expression level of miR-137 was significantly down-regulated in cervical cancer tissues and cell lines, as compared to para-cancerous tissues and H8 cells (all P<0.05). The over-expression of miR-137 significantly inhibited cell proliferation, migration and invasion of C33A and HeLa cells (all P<0.05). Moreover, Wnt5a was identified as a target of miR-137 by luciferase reporter gene assay. Furthermore, Wnt5a over-expression, to a certain degree, attenuated the suppressive effects of miR-137 on the proliferation, migration and invasion of C33A and HeLa cells. Conclusion：miR-137 can inhibit the proliferation, migration and invasion of cervical cancer cells via targeting Wnt5a, which may be an effective target for the treatment of cervical cancer.

东莞市医疗卫生科技计划资助项目（No.2016105101012）