[摘要] 目的：探讨烯醇化酶1（enolase 1, ENO1）表达水平对肺癌PC14 细胞增殖、凋亡及迁移能力的影响。方法：构建ENO1 过表达载体-pcDNA3.1/ENO1,利用脂质体LipofectamineTM 2000 转染对数生长期肺癌细胞PC14，G418 筛选ENO1 稳转细胞株。用CCK-8 法、划痕愈合实验和流式细胞术分别检测过表达ENO1 对PC14 细胞增殖、迁移和凋亡的影响。结果：成功构建了ENO1 过表达细胞模型，与PC14-vehicle 和野生型PC14 细胞相比，PC14-ENO1 细胞中ENO1 mRNA及蛋白表达水平均显著升高（均P<0.05）。PC14-ENO1 细胞增殖能力显著高于PC14-vehicle 和PC14 细胞，差异均有统计学意义（均P<0.05）；PC14-ENO1 细胞的相对迁移率明显高于pcDNA3.1-vehicle 细胞的相对迁移率[ （13.26±1.13）% vs（8.46±1.11）%、（7.86±1.00）%，均P< 0.05]，PC14-ENO1、PC14-vehicle 和PC14 细胞的凋亡率差异无统计学意义（均P > 0.05）。结论：ENO1 过表达促进肺癌PC14 细胞的增殖和迁移能力。

[Abstract] Objective: To investigate the effect of enolase 1 (ENO1) expression on proliferation, apoptosis and migration of lung cancer PC14 cells. Methods: ENO1 over-expression vector-pcDNA3.1/ENO1 was constructed and transfected into PC14 cells at logarithmic growth phase with liposome LipofectamineTM 2000. G418 was used to screen PC14 cells that stably expressing ENO1. The effects of ENO1 over-expression on proliferation, migration and apoptosis of PC14 cells were detected by CCK-8 method, scratch-healing assay and flow cytometry, respectively. Results: The ENO1 over-expression cell model was successfully constructed. Compared with PC14-vehicle and wild-type PC14 cells, the mRNA and protein expression levels of ENO1 in PC14-ENO1 cells were significantly elevated (all P<0.05), and the proliferation of PC14-ENO1 cells was significantly increased (all P<0.05). The relative mobility of PC14-ENO1 cells was significantly higher than that of pcDNA3.1-vehicle cells and wild-type PC14 cells ([13.26±1.13]% vs [8.46±1.11]%,[7.86±1.00]%, both P<0.05). There was no significant difference in apoptotic rate among PC14-ENO1, PC14-vehicle and PC14 cells (all P > 0.05) Conclusion: Over-expression of ENO1 promotes proliferation and migration of lung cancer PC14 cells.

国家自然科学基金资助项目（No.81772485）