[摘要] 目的：检测非编码RNA snord105b 在胃癌患者组织和血清及胃癌细胞中的表达水平及其与胃癌患者临床病理特征的相关性，并分析其对多种胃癌细胞增殖能力的影响。方法：收集2016 至2017 年河北医科大学第四医院普外三科未经放化疗的胃癌患者手术切除癌组织及相应的癌旁组织样本120 例、术前胃癌患者外周血50 例、健康献血者外周静脉血30 例，以及5 种胃癌细胞系SGC-7901、AGS、MGC-803、BGC-823、HGC-27 和胃黏膜正常上皮细胞GES-1。采用qPCR方法检测胃癌患者组织、血清和胃癌细胞系中snord105b 的表达水平，分析其与患者临床病理特征的相关性。通过MTS检测敲低或者过表达snord105b 对4 种胃癌细胞体外增殖能力的影响。结果：胃癌组织、血清及细胞系中snord105b 的表达水平显著高于癌旁组织、正常献血者血清及GES-1 细胞（均P<0.05），并且胃癌组织中snord105b 的表达水平与患者年龄、肿瘤大小、分化程度、TNM分期明显相关（P<0.05）；在4 种胃癌细胞中，敲低snord105b，胃癌细胞的增殖能力显著低于对照组，而过表达snord105b，胃癌细胞增殖能力则高于对照组，差异均具有统计学意义（均P<0.05）。结论：非编码snord105b 在胃癌组织、血清和细胞系中表达明显异常，与患者的年龄、肿瘤大小、分化程度及TNM分期密切相关，其可促进多种胃癌细胞的增殖能力，可能成为胃癌早期诊断及预后判断的分子标志物。

[Abstract] Objective: To detect the expression of non-coding RNA snord105b in gastric cancer (GC) tissues, sera and cell lines, and its correlation with clinicopathological characteristics of patients with GC as well as its effect on the proliferation of GC cells. Methods:One hundred and twenty pairs of GC tissues and corresponding para-cancerous tissues from patients, who underwent surgery at Department of Surgery, the Fourth Hospital of Hebei Medical University between 2016 and 2017, were collected for this study. The presurgical sera samples from GC patients (n=50) and peripheral venous blood samples from healthy donors (n=30), as well as five gastric cancer cell lines (SGC-7901, AGS, MGC-803, BGC-823, HGC-27) and gastric mucosa normal epithelial GES-1 cells were also obtained. qPCR assay was adopted to detect the expression of snord105b in GC tissues, sera and cell lines. The correlation between snord105b and patients’clinicopathological features was investigated. MTS assay was adopted to detect the effect of snord105b silence or over-expressionon in vitro proliferation of four GC cells. Results: qPCR assay demonstrated that the expression of snord105b in GC tissues, sera and cell lines were significantly higher than that of para-cancerous tissues, sera from healthy donors and GES-1 cells (all P<0.05). Expression level of snord105b was obviously associated with age,tumor size, differentiation and TNM stages of patients (all P<0.05). MTS assay demonstrated that knockdown of snord105b could suppress the proliferation of GC cells (P< 0.05), while forced-expression of snord105b could promote the proliferation of GC cells (P< 0.05). Conclusion: non-coding RNA snord105b aberrantly expressed in GC tissues, sera, and cells, and its expression was obviously correlated with patients’age, tumor size, differentiation and TNM stages. Snord105b could significantly promote the proliferation of GC cells, which may be used as a potential clinical biomaker for early diagnosis and prognosis of GC.

国家自然基金资助项目（No.81673642, No.81502032, No.81772550）；河北省卫生厅科研基金资助项目（No.20170146）