[摘要] 目的: 探讨石斛酚对人成骨肉瘤U20S 细胞增殖、迁移及侵袭的抑制作用及其分子机制。方法：用不同质量浓度（10、25、50、75、100、150 μmol/L）的石斛酚处理U20S 细胞24、48 h 后，用CCK-8 法检测石斛酚对U20S 细胞增殖能力的影响。Transwell 实验检测25、50 μmol/L的石斛酚对U20S 细胞迁移、侵袭能力的影响，在脂多糖（LPS）诱导U20S 细胞炎症反应的基础上，再以石斛酚处理，qPCR、WB法分别检测U20S细胞中NF-κB（p65）、TNF-α、IL-6、PRL-3 mRNA和蛋白的表达水平。结果：不同质量浓度的石斛酚作用不同时间对肉瘤细胞U20S 增殖均具有抑制作用（P<0.05 或P<0.01）；25、50 μmol/L的石斛酚能够明显抑制骨肉瘤U20S细胞的迁移及侵袭能力（均P<0.01），同时能够抑制细胞中NF-κB、TNF-α、IL-6、PRL-3 等蛋白的表达（P<0.05 或P<0.01）；LPS诱导后，U20S 细胞中NF-κB、TNF-α、IL-6、PRL-3 mRNA和蛋白表达水平均显著升高（均P<0.01），25、50 μmol/L的石斛酚处理后均能够降低U20S细胞中NF-κB、TNF-α、IL-6、PRL-3 mRNA和蛋白的表达水平（P<0.05 或P<0.01）。结论：石斛酚对骨肉瘤U20S细胞增殖、迁移和侵袭具有抑制作用，其作用机制可能与调控NF-κB/PRL-3 信号通路有关。

[Abstract] Objective: To study the inhibitory effect of gigantol on proliferation, migration and invasion of human osteosarcoma U20S cells and to explore the mechanism. Methods: After being treated with different concentrations (10, 25, 50, 75, 100, 150 μmol/L) of gigantol for 24 and 48 h, the proliferation of U20S cells was detected by CCK-8 assay. Transwell assay was used to detect the effects of 25 μmol/L and 50 μmol/L gigantol on the migration and invasion abilities of U20S cells. The lipopolysaccharide (LPS) was used to induce inflammatory reaction in U20S cells before gigantol treatment; qPCR and WB were used to detect the mRNA and protein expressions of NF-κB (p65), TNF-α, IL-6 and PRL-3, respectively. Results: Different concentrations of gigantol could all inhibit the proliferation of sarcoma U20S cells at different time (P<0.05 or P<0.01). The 25 μmol/L and 50 μmol/L of gigantol could significantly inhibit the migration and invasion of osteosarcoma U20S cells (all P<0.01); at the same time, it could inhibit the protein expressions of NF- κB, TNF- α , IL-6 and PRL-3 (P<0.05 or P<0.01). After LPS induction, the mRNA and protein expressions of NF- κB, TNF- α , IL-6 and PRL-3 in U20S cells were significantly increased (all P<0.01); however, the consequent treatment with gigantol (25 and 50 μmol/L) reversed the effects of LPS on U20S cells obviously (P<0.05 or P<0.01). Conclusion:Gigantol can inhibit the proliferation, migration and invasion of osteosarcoma U20S cells, and its mechanism may be related to the regulation of NF-κB/PRL-3 signaling pathway.

河南省科技发展计划资助项目（No.142102310453）