目的：探究miR-130a-3p 通过HGF/MET信号通路调控上皮间质转化（epithelial-mesenchymal transition，EMT）影响乳腺癌细胞侵袭转移的分子机制。方法：收集承德医学院附属医院2018 年1 月至10 月收治的22 例乳腺癌患者癌组织和配对癌旁组织标本，乳腺癌细胞系（MCF-7、MDA-MB-231 和MDA-MB-453）和正常乳腺上皮细胞MCF10A来自承德医学院基础研究所，然后采用qPCR检测组织和细胞系中miR-130a-3p 的表达情况；将实验分为对照组、miR-130a-3p mimics 组、miR-130a-3p inhibitor组、PHA665752（MET小分子抑制剂）转染组及共转PHA665752+miR-130a-3p inhibitor 组，然后采用CCK-8 法和Transwell 实验分别检测MCF-7 细胞增殖活力、侵袭和迁移能力；WB实验检测MCF-7 细胞EMT和HGF/MET信号通路相关蛋白的表达情况；此外，采用双荧光素酶报告基因检测miR-130a-3p 与MET之间的靶向关系。结果：miR-130a-3p 在乳腺癌组织和细胞系中呈低表达；过表达miR-130a-3p 可抑制MCF-7 细胞增殖、侵袭、迁移和EMT；而抑制miR-130a-3p 出现相反的结果。双荧光素酶报告基因结果证实miR-130a-3p 靶向下调MET的表达水平，且miR-130a-3p 负调控HGF/MET信号通路的表达；进一步实验证明，miR-130a-3p 通过阻断HGF/MET信号通路抑制MCF-7 细胞增殖、侵袭、迁移和EMT。结论：miR-130a-3p 通过阻断HGF/MET信号通路抑制MCF-7 细胞EMT过程，进而抑制MCF-7 细胞侵袭转移。

Objective: To explore the molecular mechanism of miR-130a-3p regulating epithelial mesenchymal transition (EMT) to affect the invasion and metastasis of breast cancer cells through HGF/MET pathway. Methods: A total of 22 pairs of cancer tissues and adjacent normal tissues from breast cancer patients, who were admitted to Affiliated Hospital of Chengde Medical College from January 2018 to October 2018, were collected for this study; in addition, breast cancer cell lines (MCF-7, MDA-MB-231 and MDA-MB-453) and normal breast epithelial cells MCF10A were obtained from the Institute of Basic Sciences, Chengde Medical College. And then, the expression of miR-130a-3p in tissues and cell lines were detected by qRT-PCR. The experiment cells were divided into control group, miR-130a-3p mimics group, miR-130a-3p inhibitor group, PHA665752 (a small-molecule MET inhibitor) transfection group and PHA665752+miR-130a-3p inhibitor co-transfection group. CCK-8 assay and Transwell assay were performed to detect the proliferation,invasion and migration of MCF-7 cells, respectively. The expressions of EMT and HGF/MET signaling pathway related proteins in MCF-7 cells were detected by WB. In addition, the targeted relationship between miR-130a-3p and MET was verified by Dual luciferase reporter gene assay. Results: miR-130a-3p was down-regulated in breast cancer tissues and cell lines. Over-expression of miR-130a-3p could suppress the proliferation, invasion, migration and EMT of MCF-7 cells, while knockdown of miR-130a-3p had the opposite results. The results of Dual luciferase reporter gene assay indicated that miR-130a-3p targetedly down-regulated the expression of MET, and miR-130a-3p negatively regulated the expression of HGF/MET signaling pathway. Further experiments confirmed that miR-130a-3p inhibited the proliferation, invasion, migration and EMT of MCF-7 cells by blocking HGF/MET signaling pathway. Conclusion:miR-130a-3p suppresses the EMT of MCF-7 cells via blocking HGF/MET signaling pathway, thereby repressing the invasion and metastasis of MCF-7 cells.

承德市科技支撑计划项目资助（No. 201701A080）