目的：探讨黑色素瘤相关抗原A9（MAGE-A9）、MAGE-A11-和Ki67 在喉鳞状细胞癌组织中的表达，并分析其与喉鳞状细胞癌患者临床病理学特征及预后的关系。方法：选取河北医科大学第四医院2012-2014 年住院手术切除的喉鳞状细胞癌组织及相应癌旁组织标本各73 例，同时选取该院3 例前列腺癌住院患者去势治疗术后的睾丸组织作为阳性对照，应用免疫组织化学法检测喉鳞状细胞癌组织及相应癌旁组织中MAGE-A9、MAGE-A11 和Ki67 蛋白的表达水平。结果：喉鳞状细胞癌组织中MAGE-A9、MAGE-A11 蛋白和Ki67 的表达率[47.94%（35/73）]、[49.32%（36/73）]和[46.58%（34/73）]均显著高于相应的癌旁组织[0（0/75）（P<0.01）]，MAGE-A9 和MAGE-A11 蛋白的表达与喉鳞状细胞癌患者的临床分期和淋巴转移有关（P<0.05），Ki67LI 表达与喉鳞状细胞癌患者的肿瘤大小、临床分期和淋巴转移有关（P<0.05）。相关性分析显示，MAGE-A9 和MAGE-A11 蛋白表达均与Ki67 指数呈正相关（r=0.258，P＝0.027；r=0.672，P=0.001）。Kaplan-Meier 生存曲线分析显示，MAGE-A9、MAGE-A11 和Ki67LI蛋白高表达阳性患者的生存率均显著低于低表达的患者，而且MAGE-A9 和Ki67LI 均高表达或MAGE-A11 和Ki67LI 均高表达患者的生存期均显著低于其单一高表达或均低表达的患者（P<0.05 或P<0.01）。单因素和多因素Cox回归模型分析进一步提示，MAGE-A9 蛋白和MAGE-A11 蛋白是喉鳞状细胞癌患者总体生存的独立预后因素（P<0.05）。结论：MAGE-A9、MAGE-A11 和Ki67 是喉鳞状细胞癌的肿瘤相关抗原，其可作为预测喉鳞状细胞癌患者的预后指标。

Objective: To explore the expressions of melanoma antigen (MAGE) -A9, -A11 and Ki67 in laryngeal squamous cell carcinoma (LSCC) tissues, and to analyze their correlation with clinicopathological features and the prognosisof LSCC patients. Methods: A total of 73 pairs of LSCC tissuesand corresponding para-cancerous tissues resected from LSCC patients, who were treated at the Fourth Hospital of Hebei Medical University from 2012 to 2014,were collected for this study. At the same time, testicular tissues from 3 patients with prostate cancer after castration were selected as positive control. The protein expressions of MAGE-A9, MAGE-A11 and Ki67 in LSCC tissues and its para-cancerous tissues were detected by immunohistochemistry. Results: The expression rates of MAGEA9,MAGE-A11 protein and Ki67 in LSCC tissues were 47.94% (35/73), 49.32% (36/73) and 46.58% (34/73) respectively, which were significantly higher than those in para-cancerous tissues. The protein expressions of MAGE-A9 and MAGE-A11 were correlated with clinical stage and lymphatic metastasis of LSCC (P<0.05). The expression of Ki67LI was correlated with tumor size, clinical stage and lymphatic metastasis of LSCC (P<0.05). The correlation analysis showed that the expressions of MAGE-A9 and MAGE-A11 were positively correlated with Ki67 (r=0.258, P＝0.027; r=0.672, P=0.001). Kaplan-Meier survival curve analysis showed that the survival rates of patients with high expression of MAGE-A9 protein (P=0.009), MAGE-A11 protein (P=0.031) and Ki67LI (P=0.040) were signifi‐cantly lower than those with low expressions. And the survival time of patients with both high expressions of MAGE-A9 and Ki67LI (P=0.001) or both high expressions of MAGE-A11 and Ki67 (P=0.001) was significantly shorter than that of patients with low expression (both or single). Univariate and multivariate Cox regression analysis further indicated that MAGE-A9 protein (P=0.028) and MAGE-A11 protein (P=0.042) were independent prognostic factors for overall survival of LSCC patients. Conclusion: MAGE-A9,MAGE-A11 and Ki67 are tumor-associated antigens of LSCC, which can be used as prognostic indicators for LSCC.

河北省财政支撑项目资助（No. [2016]361006）