目的：探究lncRNA01296 在食管癌组织中的表达及其对食管癌TE-2细胞增殖和迁移的影响。方法：收集2017年1 月至2018年9月承德医学院附属医院肿瘤科收治的36例食管癌组织及相应的癌旁组织，并培养人正常食管上皮细胞（HEEC）及人食管癌细胞系ECA109、TE-1 及TE-2。用qPCR检测癌组织及ECA109、TE-1 及TE-2 细胞中lncRNA01296、小核核糖核蛋白A(SNRPA)及神经生长因子（NGF）mRNA的表达水平。重组慢病毒干扰载体转染食管癌细胞系及相应对照细胞系，分别分为干扰1组、干扰2组及对照组，WB实验检测转染后细胞SNRPA 及NGF 蛋白的表达水平，MTS 实验检测转染后TE-2 细胞的增殖能力，Transwell实验检测TE-2 细胞侵袭和迁移能力。结果：lncRNA01296、SNRPA及NGF mRNA在食管癌组织及细胞系中呈高表达（均P<0.01），且lncRNA01296、SNRPA及NGF mRNA在低分化的TE-2细胞中表达高于其他癌细胞（均P<0.05）。干扰1组和干扰2组lncRNA01296、NGF mRNA表达水平明显低于对照组（均P<0.01），而SNRPAmRNA表达水平与对照组无明显差异（P>0.05）；干扰1组和干扰2 组SNRPA及NGF蛋白表达水平明显低于对照组（均P<0.01）。敲减48、72 h后，干扰1组及干扰2组TE-2细胞相对增殖能力明显低于对照组（P<0.05或P<0.01）；对照组、干扰1组及干扰2组侵袭细胞数分别为（72.0±6.3）、（36.6±4.3）及（33.9±3.7）个，迁移细胞数分别为（85.2±9.9）、（47.5±8.1）及（43.8±6.5）个，干扰1 组及干扰2 组侵袭及迁移细胞数显著低于对照组（均P<0.01）；结论：lncRNA01296可通过上调SNRPA表达促进NGF介导的食管癌细胞的增殖和迁移，为临床食管癌诊断和治疗提供新的靶点。

Objective: To investigate the expression of lncRNA01296 in esophageal cancer (EC) tissues and its effect on the proliferation and migration of EC TE-2 cells. Methods: A total of 36 pairs of esophageal cancer tissues and corresponding para-cancerous tissues were collected from EC patients admitted to the Department of Thoracic Surgery, Affiliated Hospital of Chengde Medical College from January 2017 to September 2018. The human normal esophageal epithelial (HEEC) cells and human esophageal cancer cell lines ECA109, TE-1 and TE-2 were cultured. qPCR was used to detect the mRNA expressions of lincRNA01296, SNRPA (small nuclear ribonucleoprotein A) and NGF (nerve growth factor) in EC tissues and cells. Recombinant lentiviral interference vectoror control vector were used to transfect EC cell lines, as sh-lncRNA01296#1,#2 and Mock groups. WB was used to detect the protein expressions of SNRPA and NGF in transfected cells. MTS assay was used to detect cell proliferation, and Transwell assays were used to detect cell invasion and migration of TE-2 cells after transfection. Results: The mRNA expressions of lncRNA01296, SNRPA and NGF were significantly increased in esophageal cancer tissues and cell lines (all P<0.01), and these expressions in poorly differentiated TE-2 cells were higher than those in highly differentiated ECA109 and TE-1 cells (all P<0.05). The mRNA expressions of lncRNA01296 and NGF in sh-lncRNA01296#1 and sh-lncRNA01296#2 groups were significantly lower than those in Mock group (all P<0.01), while the mRNAexpression of SNRPAshowed no statistical difference among three groups (P>0.05). The protein expressions of lncRNA01296 and NGF in sh-lncRNA01296#1 and sh-lncRNA01296#2 groups were significantly lower than those in Mock group (all P<0.01). The relative proliferation ability of cells in sh-lncRNA01296#1 and shlncRNA01296# 2 groups was significantly lower than that of Mock group at 48 and 72 h after transfection (P<0.05 or P<0.01). The number of invasive cells was (72.0±6.3), (36.6±4.3) and (33.9±3.7) in Mock, sh-lncRNA01296#1 and sh-lncRNA01296#2 groups, respectively; and the number of migrated cells was (85.2±9.9), (47.5±8.1) and (43.8±6.5), respectively, indicating that the numbers of invasive and migrated cells in sh-lncRNA01296#1 and sh-lncRNA01296#2 groups were significantly less than those in Mock group(all P<0.01). Conclusion:lncRNA01296 can up-regulate SNRPA expression to promote NGF-mediated proliferation and metastasis of EC cells, which may provide new target for the diagnosis and treatment of esophagealcancer.

承德市科技局科技计划资助项目（No.20168002）