目的：通过液相芯片技术评价消积饮联合化疗对晚期结直肠癌患者血液细胞因子表达谱的影响。方法：回顾性分析 2018年1月1日至2018年12月31日来自广东省中医院大学城分院肿瘤科的14例符合纳入标准的晚期结直肠癌患者，根据治疗分 为化疗组（n=7）和联合治疗组（n=7），化疗组给予5-氟尿嘧啶+亚叶酸钙+奥沙利铂（FOLFOX）治疗，联合治疗组给予消积饮+FOLFOX 治疗，评价两组患者治疗6程后疗效， 并在每 2 个疗程后采用液相芯片技术检测患者外周静脉血清中细胞因子表达谱。结果： 14 例患者共接受了84程治疗，生存分析显示联合治疗组和化疗组中患者的PFS、OS因样本量不足不能进行对比，但联合治疗组较化 疗组的PFS及OS均有延长的趋势（PFS： 10 vs 6个月，OS： 17 vs 12个月）；不良反应方面，两组出现白细胞减少、腹泻、恶心、末梢神 经炎及脱发等不良反应发生例数相当，但联合治疗组较化疗组不良反应程度略轻。化疗组患者血清中IL-2和脑源性神经营养血液 细胞因子（Brain derived neurotrophic blood cytokines，BDNF）的浓度高于联合治疗组（P<0.05）。比较治疗前后不同采血点细胞因子 浓度，患者治疗2程后化疗组的IL-2浓度高于联合治疗组（P<0.05）。在不同用药周期，共有19个细胞因子出现联合治疗组高于化 疗组的趋势。结论：消积饮联合FOLFOX方案可能是晚期结直肠癌的一种值得探索的治疗方案，液相芯片分析其机制可能与降低 患者血清中细胞因子IL-2和BDNF水平有关。

Objective: To investigate the effects of XIAOJI Decoction combined with FOLFOX chemotherapy on serum cytokine expression profile in patients with advanced colorectal carcinoma by liquid chip technology. Methods: Fourteen patients with advanced colorectal carcinoma, who met the inclusion criteria and were treated in the Department of Oncology, Higher Education Mega Center Hospital of Guangdong Provincial Hospital of Traditional Chinese Medicine during January 1, 2018 and December 31, 2018 were retrospectively analyzed in this study. The patients were divided into chemotherapy group (n=7, treated with 5-Fluorouracil + Calcium Folic Acid+Oxaliplatin (FOLFOX)) and combined treatment group (n=7, treated with XIAOJI Decoction + FOLFOX) according to therapeutic scheme. The curative efficacy was evaluated after 6 treatment courses. The expression profile of cytokines in blood serum of patients was examined by liquid chip technology after every 2 courses. Results: Fourteen patients received a total of 84 cycles of therapy. Survival analyses showed that the progress-free survival time (PFS) and overall survival time (OS) of two groups couldn't be compared due to insufficient samples, although the combined treatment group had longer PFS (10 months vs 6 months) and OS (17 months vs 12 months) than the chemotherapy group.As to adverse reactions, the rates of leucopenia, diarrhea, nausea, peripheral neuritis and alopecia in two groups were comparable, while the severity in combined treatment group were lighter than that in chemotherapy group. In comparison with the combined treatment group, concentrations of serum BDNF and IL-2 were statistically higher in the chemotherapy group (P<0.05). By comparing the cytokine concentrations at different collection time points before and after the treatment, it showed that the concentration of serum IL-2 in chemotherapy group was higher than that in combined treatment group after 2 courses of treatment (P<0.05). In total, there were 19 cytokines showed a tendency to be higher in combined treatment group than chemotherapy group during different treatment periods. Conclusion: Combined treatment of XIAOJI Decoction with FOLFOX for advanced colorectal carcinoma is a treatment option worth exploring, and liquid chip analysis showed that the mechanism may be related to the reduction of serum LI-2 and BDNF levels in patients.

广东省科学技术厅科技计划项目资助（No. 2017ZC0151）