目的：探讨过表达叉头框转录因子FOXK2 对人卵巢癌SK-OV-3 细胞增殖、迁移、侵袭、黏附等的影响及相关分子机制。方法：将FOXK2 基因编码序列克隆到慢病毒表达载体，在HEK293T细胞中包装慢病毒并感染人卵巢癌SK-OV-3 细胞，用qPCR和Western blotting 检测过表达效果，用CCK-8 法、细胞划痕愈合、Transwell 和细胞黏附实验分别检测细胞的增殖、迁移、侵袭和黏附能力，用qPCR 检测细胞上皮间质转化（epithelial-mesenchymal transition, EMT）标志物表达水平。结果：成功构建了FOXK2 基因过表达载体并包装成慢病毒，将该慢病毒成功感染了SK-OV-3 细胞并使FOXK2 的表达水平显著上调（P<0.01）。过表达FOXK2 后，SK-OV-3 细胞的增殖、迁移和侵袭能力显著降低、黏附能力显著升高（P<0.05 或P<0.01），E-cadherin 和β-catenin表达水平显著升高而vimentin 和fibronection 表达水平显著降低（均P<0.01）。结论：过表达FOXK2 基因导致卵巢癌SK-OV-3 细胞的增殖、迁移和侵袭能力显著降低、黏附能力显著升高，其分子机制可能是阻止肿瘤细胞的EMT进程，FOXK2 可能是卵巢癌诊疗的一个潜在靶标。

Objective: To investigate the effects of forkhead box transcription factor (FOXK2) overexpression on the proliferation,migration, invasion and adhesion of human ovarian cancer SK-OV-3 cells and its related molecular mechanism. Methods: The open reading frame (ORF) of FOXK2 was cloned into lentivirus expression vector, which was then enveloped in HEK293T cells and transfected into human ovarian cancer SK-OV-3 cells. The overexpression efficiency was detected by qPCR andWestern blotting. The proliferation,migration, invasion and adhesion of SK-OV-3 cells were detected by CCK-8, Scratch-healing, Transwell and Cell adhesion assays respectively, and the expressions of epithelial-mesenchymal transition (EMT) markers were detected by qPCR. Results: The FOXK2 overexpression vector was constructed successfully and packaged into lentivirus, which was then transfected into SK-OV-3 cells. After transfection, the expression of FOXK2 was significantly increased (P<0.01); the proliferation, migration and invasion of SK-OV-3 cells were significantly reduced while the adhesion ability was significantly increased (P<0.05 or P<0.01); and the expression levels of E-cadherin and β-catenin were significantly increased while that of vimentin and fibronection were significantly decreased (all P<0.01). Conclusion: Overexpression of FOXK2 in SK-OV-3 cells leads to a significant decrease in proliferation, migration and invasion but increase in adhesion. The molecular mechanism may be related to the reversion of the EMT process in tumor cells,suggesting that FOXK2 may be a potential target for the diagnosis and treatment of ovarian cancer.

山东省教育厅重点基金资助项目（No. 2013B25）