目的：探讨长链非编码RNA（long noncoding RNA，lncRNA）linc01503 在食管鳞状细胞癌（esophageal squamous cell cancer，ESCC）组织和细胞中的表达及其对ESCC 细胞增殖、侵袭及上皮间质转化（epithelial-mesenchymal transition，EMT）的影响。方法：选取2012 年1 月至2014 年12 月河北医科大学第四医院收治的119 例ESCC患者的癌及癌旁组织标本，应用qPCR法检测ESCC 组织及ESCC 细胞系（Kyse150、Kyse170、Eca109、TE1 和TE13）中linc01503 的表达水平；分别用pGPU6-shRNAlinc01503转染、TGF-β1 处理ESCC 细胞，用qPCR 法检测转染前后EMT相关基因及处理前后linc01503 表达的变化，用MTS及Transwell 小室法检测linc01503 对ESCC细胞增殖及侵袭的影响。结果：linc01503 在ESCC组织和细胞系中高表达（均P<0.05），ESCC组织中linc01503 高表达与患者淋巴结转移、浸润深度、TNM分期有关联，并与生存期密切相关（均P<0.05）。TGF-β 处理促进ESCC细胞的EMT过程，同时诱导linc01503 表达明显上调。linc01503 敲低明显抑制细胞的增殖及侵袭能力（均P<0.05），同时明显增加了E-cadherin 的表达而降低了N-cadherin、vimentin 基因的表达（均P<0.05）。结论：linc01503 作为TGF-β 的下游效应基因之一，促进了ESCC细胞的增殖、侵袭及EMT过程。

Objective: To investigate the expression of lncRNA linc01503 in esophageal squamous cell carcinoma (ESCC) tissues and cell lines, as well as its effect on the proliferation, invasion and epithelial-mesenchymal transition (EMT) of ESCC cells. Methods: A total of 119 pairs of tumor tissues and corresponding para-cancerous tissues of ESCC patients were obtained from the Fourth Affiliated Hospital of Hebei Medical University between Jan. 2012 and Dec. 2014. The expression of linc01503 in ESCC tissues and cell lines (Kyse150, Kyse170, Eca109, TE1 and TE13) was detected by qPCR. The ESCC cells were transfected with pGPU6-shRNA-linc01503 or treated with TGF-β. The expressions of EMT related genes before and after transfection as well as linc01503 expression before and after TGF-β treatment were detected with qPCR. MTS and Transwell assay were performed to assess the effect of linc01503 on proliferation and invasion of ESCC cells. Results: The expression of linc01503 was significantly elevated in ESCC tissues and cell lines (all P<0.05). High expression of linc01503 was correlatedwith lymph node metastasis, depth of infiltration, TNM stage and the survival of ESCC patients (all P<0.05). Treatment with TGF-β promoted EMT of ESCC cells and induced a significant up-regulation of linc01503 expression. Knockdown of linc01503 significantly inhibited proliferation and invasion ability of ESCC cells; Meanwhile, the low expression of linc01503 increased the expression of E-cadherin but decreased the expressions of N-cadherin and vimentin (all P<0.05).Conclusion: lncRNA linc01503, as one of the downstream effect genes of TGF- β , promotes the proliferation, invasion and EMT process of ESCCcells.

河北省自然科学基金资助项目（No. H2019206664）；河北省医学科学研究重点课题计划项目（No. 20170698）；河北省人才工程培养资助项目（No.201901035）