目的：探讨lncRNA LINC00969在乳腺癌细胞增殖和迁移中的作用及其相应机制。方法: qPCR检测LINC00969在42例乳腺癌组织和对应癌旁组织（标本收集自 2017 年 9 月至 2019 年 12 月福州市第一医院普外科手术患者），以及正常乳腺细胞MCF-10A和5种乳腺癌细胞MCF-7、BT-20、MAD-MB-231、ZR-75-1、SKBR3中的表达。以过表达LINC00969质粒和空载体Vector转染乳腺癌MCF-7细胞，以qPCR验证转染效率；以CCK-8法、平板克隆和EDU实验检测细胞增殖水平，流式细胞术检测细胞周期，Western blotting实验检测细胞中PCNA、CyclinD1和MMP2、MMP9水平，划痕修复实验和Transwell实验检测细胞的迁移和侵袭。结果: 与癌旁组织比较，乳腺癌组织中LINC00969表达显著降低（P<0.05）；与乳腺细胞MCF-10A比较，5种乳腺癌细胞中LINC00969表达均显著降低（P<0.05或P<0.01），而以MCF-7细胞中最低；过表达LINC00969使MCF-7细胞的增殖、集落形成和DNA合成能力均受到显著抑制（P<0.05或P<0.01），使MCF-7细胞周期明显阻滞于G1期，使细胞的划痕愈合和侵袭能力明显降低（P<0.05或P<0.01）。过表达 LINC00969 使 MCF-7 细胞中 PCNA、CyclinD1、MMP2和MMP9的表达受到明显抑制（均P<0.05）。结论: LINC00969在乳腺癌中低表达，上调LINC00969表达可以抑制乳腺癌MCF-7细胞的增殖、迁移和侵袭，其机制可能涉及细胞周期与迁移相关蛋白表达的异常。

Objective: To investigate the effect of long non-coding LINC00969 on proliferation and invasion of breast cancer cell.Methods: Real-time Quantitative polymerase chain reaction (qPCR) was used to detect differential expression of LINC00969 in five breast cancer cell lines (MCF-7, BT-20, MAD-MB-231, ZR-75-1, and SKBR3) , normal breast cells MCF-10A, and in 42 cases breast cancer tissues and adjacent tissues. TMCF-7 cells were transfected with LINC00969 plasmid and empty vector vector vector. The trans‐fection efficiency was verified by qPCR. CCK-8, plate cloning and edu assay were used to detect cell proliferation. Flow cytometry was used to detect cell cycle. Western blotting was used to detect PCNA, CyclinD1, MMP2 and MMP9. Scratch repair test and Transwell test were used to detect cell migration and invasion. Results：Compared with the adjacent tissues, LINC00969 expression in breast can‐cer tissues was significantly decreased (P<0.05); compared with breast cancer cells MCF-10A, LINC00969 expression in five breast cancer cells was significantly decreased (P<0.05), and the lowest was in MCF-7 cells; overexpression of LINC00969 significantly inhib‐ited the proliferation, colony formation and DNA synthesis of MCF-7 cells (all P<0.05), making MCF-7 cell cycle clear.The ability of wound healing, migration and invasion of the cells were significantly reduced (P<0.05). Overexpression of LINC00969 significantly in‐hibited the expression of PCNA, cyclinD1, MMP2 and MMP9 (all P<0.05). Conclusion: LINC00969 is low expressed in breast cancer.Overexpression of LINC00969 can inhibit proliferation and migration of breast cancer cell,the mechanism may be related to the abnor‐mal expression of cell cycle and migration related proteins.

福建省自然科学基金资助项目(No.2019J01540)