目的：探讨长链非编码RNASNHG1（long non-coding RNASNHG1，lncRNASNHG1）在子宫内膜癌组织中的表达水 平，分析其在子宫内膜癌中的作用机制及其临床意义。方法：采用NCBI-GEO和TCGA数据库分析SNHG1在子宫内膜癌中的 表达水平。收集2016年1月至2019年3月天津医科大学中新生态城医院手术切除的53例子宫内膜癌组织和41例正常子宫内膜 组织标本，以及子宫内膜癌细胞系Ishikawa和HEC-1A、正常子宫内膜细胞ESC， 用qPCR检测组织和细胞中SNHG1的表达水平， 并分析SNHG1表达水平与患者的临床病理特征的相关性。用MTT、Annexin V/PI染色法流式细胞术检测SNHG1对HEC-1A细 胞增殖能力和凋亡水平的影响， 用Transwell小室法检测HEC-1A细胞的迁移及侵袭能力。用StarBase预测并用qPCR和Western blotting验证SNHG1与RELA的调控关系，用双荧光报告基因实验、qPCR验证SNHG1影响NF-κB信号通路。结果：SNHG1在 子宫内膜癌组织中的表达水平显著高于正常子宫内膜组织（P<0.01），其表达水平与肿瘤大小、TNM分期、组织学分级和淋巴结转 移相关联（均P<0.05）；Ishikawa和HEC-1A细胞中SNHG1的表达水平显著高于ESC细胞（均P<0.01）。过表达SNHG1可明显促 进HEC-1A细胞的增殖、迁移和侵袭能力，并抑制HEC-1A细胞的凋亡（均P<0.01）。SNHG1通过促进RELA的表达激活了NF-κB 信号通路，并促进下游靶基因IL-6和CCL19的表达（均P<0.01）。结论：子宫内膜癌中高表达的SNHG1通过上调RELA激活 NF-κB信号通路发挥其促癌的作用。

Objective: To investigate the expression level of lncRNA (long non-coding RNA) SNHG1 in endometrial cancer tissues, and to analyze its mechanism of action as well as its clinical significance. Methods: NCBI-GEO and TCGA database were used to analyze the expression level of SNHG1 in endometrial cancer. A total of 53 cases of endometrial cancer tissue samples and 41 cases of normal endometrial tissuesampleswerecollectedfromJanuary 2016 to March 2019atZhongxin Ecocity Hospital of Tianjin Medical University;inaddition,endometrialcancercelllinesIshikawaandHEC-1AaswellasnormalendometrialESCcellswerealsocollected for thisstudy. qPCR was used to detecttheexpressionlevelofSNHG1intissuesandcells,anditscorrelation with the clinical characteristics of patients were statistically analyzed. The effect of SNHG1 on cell proliferation and apoptosis of HEC-1A cells was measured by MTT assay and Annexin V/PI double staining Flow cytometry, respectively. The migration and invasion of HEC-1A cells were measured by Transwell assay. StarBase was used to predict the regulatory relationship between SNHG1 and RELA, which was then verified by qPCR and Western blotting. Dual fluorescent reporter gene system and qPCR were adopted to identify the influence of SNHG1 on NF-κB pathway. Results: The expression of SNHG1 was significantly up-regulated in endometrial cancer tissues compared with normal endometrial tissues (P<0.01), and its expression was related to tumor size, TNM staging, histological grade and lymph node metastasis (all P<0.05). The expression level of SNHG1 in Ishikawa and HEC-1A cells was significantly higher than that in ESC cells (all P<0.01). Overexpression of SNHG1 notably promoted the proliferation, migration and invasion and inhibited cell apoptosis of HEC-1A cells. By promoting the expression of RELA, SNHG1 activated the NF-κB pathway and promoted the expressions of downstream gene IL-6 and CCL19 (all P<0.01). Conclusion: Up-regulated SNHG1 in endometrial cancer functions as an oncogene by activating the NF-κB pathway through promoting the RELAexpression.

国家自然科学基金资助项目（No.81902870）；天津医科大学肿瘤医院检验专项基金（No.Y1701）