目的：探讨长链非编码RNA（long non-coding RNA，lncRNA）HOTTIP在子宫内膜癌患者组织中的表达及其临床意义。方法:选取 2012 年 4月至2014年4月在河南省新乡市中心医院接受手术治疗的109例子宫内膜癌患者的癌组织及其相应癌旁组织（距离癌缘5 cm以上）标本，用qPCR法检测子宫内膜癌和癌旁组织中HOTTIP的表达，所有患者于术后第1天开始随访，随访截止日期 2019 年 4 月 30 日，随访终点事件为死亡，记录患者生存时间。结果:HOTTIP 在子宫内膜癌组织中相对表达量为（2.55±0.21），高于癌旁组织中的（1.03±0.16）（t=60.631，P<0.01）；HOTTIP在不同FIGO分期、组织学分级、肌层浸润深度、是否发生淋巴血管间隙浸润及淋巴结转移间表达量存在差异（均P<0.05）。Kaplan-Meier生存分析结果显示，低表达组患者5年生存率和生存时间均高于高表达组[78.57% vs 37.04%，（70.67±4.94）vs（42.14±3.65）个月（] χ2=12.839，P<0.01）。Cox比例风险回归模型分析结果显示，FIGO 分期[HR=2.248（95%CI：1.034～4.887）]、肌层浸润深度[HR=3.055（95%CI：1.668～5.592）]、淋巴结转移[HR=3.811（95%CI：1.786～8.131）]和HOTTIP表达[HR=2.649（95%CI：1.026～6.842）]均是子宫内膜癌患者预后的独立影响因素。结论:lncRNA HOTTIP在子宫内膜癌组织中高表达，与患者恶性进展有关，是患者预后的独立影响因素。

Objective: To investigate the expression and clinical significance of long non-coding RNA (lncRNA) HOTTIP in tissues of patients with endometrial carcinoma. Methods: A total of 109 cases of patients with endometrial carcinoma who underwent surgery in Xingxiang Central Hospital from April 2012 to April 2014 were selected. The endometrial carcinoma tissue and its corresponding adjacent tissue (more than 5 cm from the cancer margin) were obtained. The expressions of HOTTIP in endometrial carcinoma and adjacent tissues were detected by qRT-PCR. All patients were followed up from the first postoperative day. The follow-up deadline was April 30, 2019. The end-point event was death and the patient's survival time was recorded. Results: The relative expression level of HOTTIP in endometrial carcinoma tissues was (2.55±0.21), which was higher than that in the adjacent tissue (1.03±0.16) (t=60.631,P<0.01). The differences of the relative expression levels of HOTTIP in endometrial carcinoma tissues between different FIGO stage,histological grade, depth of myometrial invasion, lymphatic vascular infiltration status and lymph node metastasis were statistically significant (all P<0.05). Kaplan-Meier survival analysis showed that the 5-year survival rate and the survival time in the low expression group were higher than those in the high expression group [78.57% vs 37.04%, (70.67±4.94) months vs (42.14±3.65) months], the difference was statistically significant (χ2 =12.839, P<0.01). Cox proportional hazards regression model analysis showed that the FIGO stage [HR=2.248 (95%CI: 1.034-4.887)], myometrial invasion depth [HR=3.055 (95%CI: 1.668-5.592)], lymph node metastasis [HR=3.811 (95%CI: 1.786-8.131)] and the expression of HOTTIP [HR=2.649 (95%CI: 1.026-6.842)] were all independent influence factors for the prognosis of patients with endometrial carcinoma. Conclusion: lncRNA HOTTIP is highly expressed in endometrial carcinoma tissues and associated with malignant progression of patients. It is an independent influencing factor for patients’prognosis.

吴 阶 平 自 然 科 学 基 金 资 助 项 目（ No. 320.6750.14109 ）