目的：检测miR-126在口腔鳞状细胞癌（oral squamous cell carcinoma，OSCC）中的表达，分析其表达与患者临床病理特征及预后的关系，探讨其过表达对Tca8113细胞恶性生物学行为的影响。方法: 选取2016年6月至2018年6月在郑州大学第一附属医院手术治疗的62例OSCC患者的癌及癌旁组织标本，以及人舌鳞癌细胞株Tca8113和人口腔角质细胞株HOK，用qPCR法检测癌组织及细胞中miR-126的表达，分析 miR-126 表达与患者临床病理特征和预后的关系。利用脂质体转染技术，将miR-126 mimics、miR-NC质粒分别转染进Tca8113细胞，分别采用MTT法、流式细胞术及Trasnwell小室法检测细胞增殖、凋亡、迁移及侵袭能力，WB法检测凋亡、迁移和侵袭相关蛋白的表达水平。结果: miR-126在OSCC组织和Tca8113细胞中的表达水平明显低于癌旁组织和 HOK 细胞（均 P<0.01）。miR-126 表达与 OSCC 患者的 TNM 分期、淋巴结转移相关联（均 P<0.05），miR-126高表达患者的总生存率显著高于低表达组（P<0.05）。转染miR-126 mimics后，Tca8113细胞的增殖、迁移及侵袭能力明显降低（P<0.05或P<0.01），凋亡率明显升高（P<0.01）；细胞中Bcl-2、N-cadherin和vimentin表达水平明显降低（均P<0.01），Bax和E-cadherin表达水平明显升高（均P<0.01）。结论: miR-126在OSCC组织及Tca8113细胞中低表达，上调miR-126可抑制Tca8113细胞的增殖、迁移和侵袭能力，并促进细胞凋亡。

Objective: To detect the expression of miR-126 in oral squamous cell carcinoma (OSCC) and to analyze its correlation with clinicopathological features and prognosis of patients, as well as to explore the effect of miR-126 over-expression on the malignant biological behaviors of Tca8113 cells. Methods: A total of 62 pairs of cancer and para-cancerous tissue specimens from OSCC patients who were surgically treated in the First Affiliated Hospital of Zhengzhou University from June 2016 to June 2018 were collected for this study; in addition, human tongue squamous carcinoma Tca8113 cell line and human mouth keratinocyte HOK cell line were also selected for this study. The expression of miR-126 in cancer tissues and cells was detected by qPCR, and the relationship between miR-126 expression and clinicopathological features and prognosis of the patients was analyzed. miR-126 mimics and miR-NC plasmids were respectively transfected into Tca8113 cells by liposome transfection technology. Cell proliferation, apoptosis, migration and invasion were detected by MTT method, Flow cytometry and Transwell chamber method, respectively; and the expressions of apoptosis, migration and invasion related proteins were detected by Western blotting. Results: The expression level of miR-126 in OSCC tissues and Tca8113 cells was significantly lower than that in para-cancerous tissues and HOK cells (all P<0.01). The expression of miR-126 was associated with TNM stage and lymph node metastasis (all P<0.05), and patients with high miR-126 expression had significantly better overall survival rate than patients with low expression (P<0.05). After transfection with miR-126 mimics, the cell proliferation, migration and invasion ability significantly decreased (P<0.05 or P<0.01) while the apoptosis rate significantly increased in Tca8113 cells (P<0.01), the expression levels of Bcl-2, N-cadherin and vimentin in Tca8113 cells significantly decreased (all P<0.01), and expression levels of Bax and E-cadherin significantly increased (all P<0.01). Conclusion: miR-126 is low expressed in OSCC tissues and Tca8113 cells. Up-regulation of miR-126 inhibits cell proliferation, migration and invasion and promotes apoptosis of Tca8113 cells.

河南省 高 等 学 校 重 点 科 研 资 助 项 目（No. 20B320019）