目的：探讨长链非编码 RNA（lncRNA）HULC 在膀胱癌组织中的表达及其与患者临床病理特征的关系，以及沉默HULC对膀胱癌5637细胞增殖、凋亡、迁移与侵袭的影响。方法：选取2014年6月至2017年12月郑州人民医院手术切除的102例膀胱癌患者的癌及癌旁组织标本，以及人膀胱癌细胞株5637和人正常膀胱上皮细胞株SV-HUC-1，用qPCR法检测膀胱癌组织及细胞中HULC的表达，并分析HULC表达与膀胱癌患者临床病理特征的相关性，通过Kaplan-Meier生存曲线评估HULC对预后的影响。利用 siRNA 干扰技术将 si-HULC、si-NC 质粒转染进 5637 细胞中，分别采用 CCK-8 法、流式细胞术、划痕愈合实验及Transwell小室法检测沉默HULC对5637细胞增殖、凋亡、迁移及侵袭的影响。结果：膀胱癌组织中HULC的表达水平显著高于癌旁组织（P<0.01），HULC表达水平与患者肿瘤分级、肿瘤分期及淋巴结转移相关联（均P<0.05），HULC高表达患者的OS与PFS明显低于低表达者（均P<0.05）。5637细胞中HULC的表达水平明显高于SV-HUC-1细胞（P<0.01），沉默HULC后的5637细胞的增殖、迁移和侵袭能力均显著降低（均P<0.01）、细胞凋亡率明显升高（P<0.01）。结论：lncRNA HULC在膀胱癌组织及5637细胞中均高表达，沉默HULC表达可抑制膀胱癌细胞的增殖、迁移和侵袭能力并促进凋亡。

Objective: To investigate the expression of long non-coding RNA (lncRNA) HULC in bladder cancer tissues and its relationship with the clinicopathological features of patients, as well as the effect of silencing HULC on the proliferation, apoptosis,migration and invasion of bladder cancer 5637 cells. Methods: A total of 102 pairs of cancer tissue and adjacent normal tissue samples from bladder cancer patients who underwent surgical resection in Zhengzhou People’s Hospital from June 2014 to December 2017 were selected, as well as bladder cancer 5637 cell line and human normal bladder epithelial SV-HUC-1 cell line. The expression of HULC in bladder cancer tissues and cells was detected by qPCR, and the correlation between HULC and clinicopathological features of bladder cancer patients was analyzed. The effect of HULC on prognosis was evaluated by Kaplan-Meier survival curve. si-HUL and si-NC plasmids were transfected into 5637 cells by siRNA interference technology, and the effects of silencing HULC on proliferation, apoptosis, migration and invasion of 5637 cells were determined by CCK-8, Flow cytometry, Wound-healing assay and Transwell method, respectively. Results: The expression of HULC in bladder cancer tissues was significantly higher than that in normal tissues (P<0.05), and its expression level was correlated with tumor grade, tumor stage and lymph node metastasis (P<0.05).The OS and PFS of patients with high HULC expression were significantly lower than those with low expression (all P<0.05). The expression level of HULC in 5637 cells was significantly higher than that in SV-HUC-1 cells (P<0.01). After silencing HULC, the proliferation, migration and invasion of 5637 cells were significantly decreased (P<0.01), and the apoptosis rate was significantly increased (P<0.01). Conclusion: lncRNA HULC is highly expressed in bladder cancer tissues and 5637 cells. Silencing HULC expression can inhibit the proliferation, migration and invasion but promote apoptosis of bladder cancer cells.

河南省医学科技攻关计划资助项目（No. LHGJ20191077）