[ 摘 要 ] 目的：探讨环状 RNA circ_0091579 作为分子海绵吸附 miR-330-3p 介导环指蛋白 126（ring finger protein 126， RNF126）对结直肠癌（colorectal cancer，CRC）细胞增殖、凋亡、侵袭的影响。方法：选取 2019 年 2 月至 2020 年 5 月在大理大学 第一附属医院行手术治疗的 60 例 CRC 患者的癌组织和癌旁组织。构建 circ_0091579、miR-330-3p 的过表达或敲减的 CRC LoVo 细胞，qPCR 检测 CRC 组织和细胞中 circ_0091579、miR-330-3p 和 RNF126 的表达；MTT、Transwell、流式细胞术分别检测 细胞的增殖、侵袭、凋亡情况；生物信息学方法预测 circ_0091579 和 miR-330-3p、miR-330-3p 和 RNF126 的靶向关系并用双荧光 素酶报告实验和 RNA 免疫沉淀实验验证。结果：CRC 组织和多种细胞（HCT116、SW620、CW-2、LoVo 细胞）中，circ_0091579 和 RNF126 均高表达、miR-330-3p 均低表达（均 P<0.05）。敲减 circ_0091579 可以抑制 LoVo 细胞的增殖、侵袭而促进其凋 亡（均 P<0.05），但该影响在加入 miR-330-3p-inhibitor 后被逆转；过表达 miR-330-3p 使 LoVo 细胞增殖和侵袭能力减弱但 凋亡程度加强（均 P<0.05），该影响在加入 RNF126 后被抵消。circ_0091579、miR-330-3p 和 RNF126 之间存在靶向作用关系。 结论：circ_0091579 通过 miR-330-3p/RNF126 轴促进 LoVo 细胞增殖、侵袭而抑制其凋亡。

[Abstract] Objective: To identify the effect of circRNA circ_0091579 on the proliferation, apoptosis and invasion of colorectal cancer (CRC) cells by functioning as a molecular sponge of miR-330-3p and mediating ring finger protein 126 (RNF126). Methods: Sixty pairs of cancer tissue and adjacent normal tissue of CRC patients receiving surgical treatment in the First Affiliated Hospital of Dali University from February 2019 to May 2020 were collected. CRC LoVo cells with over-expression or knockdown of circ_0091579 and miR-330-3p were constructed. The expression of circ_0091579, miR-330-3p and RNF126 in CRC tissue and cell lines (HCT116, SW620, CW-2 and LoVo cells) was detected by qPCR. The proliferation, invasion and apoptosis were measured by MTT assay, Transwell assay and Flow cytometry, respectively. The targeting relationship between circ_0091579 and miR-330-3p, miR-330-3p and RNF126 was predicted by bioinformatics tool and verified by Dual luciferase reporter assay and RNA immunoprecipitation experiments. Results: In CRC tissues and cell lines, circ_0091579 and RNF126 were both highly expressed, and miR-330-3p was lowly expressed (all P＜ 0.05). Knockdown of circ_0091579 could inhibit the proliferation and invasion, but promote the apoptosis of CRC cells ( P＜ 0.05); however, this effect was reversed by miR-330-3p-inhibitor. Over-expression of miR-330-3p could suppress the ability of proliferation and invasion, but enhance the apoptosis of LoVo cells ( P＜0.05), and this effect was rescued after the participation of RNF126. There is a targeting relationship among circ_0091579, miR-330-3p and RNF126. Conclusion: circ_0091579 promotes the proliferation and invasion, and inhibits the apoptosis of LoVo cells via miR-330-3p/RNF126 axis.

云南省教育厅科学研究基金资助项目 （ No. 2019J0767）