[ 摘 要 ] 目的：探究环状 RNA(circular RNA, circRNA）0072088 在非小细胞肺癌（non-small cell lung cancer，NSCLC）细胞中 的生物学功能及其作用机制。方法：在公共基因芯片数据库 Gene Expression Omnibus（GEO）中下载 GSE101684 数据集，通过 GEO2R 分析得到差异基因。通过 qPCR 检测 NSCLC 细胞 H165、H358、H460、H226 和 A549 细胞中 circ_0072088 的表达水平， 随后采用 CCK-8 法和 Transwell 小室法分别检测 circ_0072088 对 NSCLC 细胞增殖、迁移和侵袭的作用。通过 CircInteractome 和 TargetScan 数据库预测 circ_0072088 与 miR-545-3p、miR-545-3p 与 STAT3 之间的靶向关系，并通过双荧光素酶报告基因实 验和 RNA 结合蛋白免疫沉淀（RNA binding protein immunoprecipitation，RIP）实验验证 circ_0072088、miR-545-3p 与 STAT3 之 间的靶向关系。结果：circ_0072088 在 NSCLC 细胞系中的表达均显著上调（均 P<0.05）。过表达 circ_0072088 促进了 NSCLC 细胞的增殖、侵袭和迁移（均 P<0.05）；敲低 circ_0072088 抑制了 NSCLC 细胞的增殖、侵袭和迁移（均 P<0.05）。miR-545-3p 是 circ_0072088 的下游靶点，可以被 circ_0072088 吸附；STAT3 是 miR-545-3p 的靶基因，可以被 circ_0072088 间接正向调控。 结论：Circ_0072088 通过调节 miR-545-3p /STAT3 轴促进 NSCLC 细胞的增殖和转移。

[Abstract] Objective: To explore the biological function and mechanism of circular RNA (circRNA) 0072088 in non-small cell lung cancer (NSCLC) cells. Method: GSE101684 data set was downloaded from Gene Expression Omnibus (GEO), and the differentially expressed genes (DEGs) were screened with GEO2R analysis. The expression of circ_0072088 in NSCLC cell lines (H165， H358， H460， H226 and A549 cells) was detected using qPCR. CCK-8 assay and Transwell chamber assays were utilized to assess cell proliferation, migration and invasion. CircInteractome and TargetScan database were used to predict the targeting relationship between circ_0072088 and miR-545-3p as well as between miR-545-3p and STAT3, which were then verified by Dual luciferase reporter gene experiment and RNA binding protein immunoprecipitation (RIP) experiment. Results: The expression of circ_0072088 in NSCLC cell lines was significantly up-regulated (P <0.05). Over-expression of circ_0072088 promoted the proliferation, invasion and migration of NSCLC cells (P <0.05); knockdown of circ_ 0072088 inhibited the proliferation, invasion and migration of NSCLC cells (P <0.05). MiR-545-3p is the downstream target of circ_0072088 and can be sponged by circ_0072088; STAT3 is the target gene of miR-545-3p, which can be indirectly and positively regulated by circ_0072088. Conclusion: Circ_0072088 promotes the proliferation and metastasis of NSCLC cells by regulating the miR-545-3p /STAT3 axis.

张 家 口 市 科 学 技 术 研 究 与 发 展 计 划 资 助 项 目（ No.1911021D-3）