目的：探讨盐酸石蒜碱（lycorine hydrochloride，LH）对肝癌HCCLM3细胞恶性生物学行为的影响及其对circASH2L/ miR-124-3p轴的调控作用。方法：将HCCLM3细胞分为不同浓度LH处理组（LH-L、LH-M、LH-H组）和Con、si-NC、si-circASH2L、 LH+pcDNA、LH+pcDNA-circASH2L组；以 CCK-8法、平板克隆形成实验、流式细胞术、细胞划痕实验和 Transwell小室实验分别 检测HCCLM3细胞的增殖、克隆形成、凋亡、迁移和侵袭；qPCR法检测HCCLM3细胞中circASH2L和miR-124-3p的表达量；双荧 光素酶报告基因实验检测 circASH2L 和 miR-124-3p 的靶向关系；WB 法检测 cleaved-caspase3、cleaved-caspase9、E-cadherin、 N-cadherin蛋白表达量。结果：与Con组比较，LH不同浓度组细胞增殖抑制率升高（P<0.05），划痕愈合率与N-cadherin蛋白水平降低（P<0.05），circASH2L的表达水平降低（P<0.05），细胞增殖抑制率降低（P<0.05），克隆形成数与侵袭细胞数增多（均P<0.05），划痕愈合率与N-cadherin蛋白水平升高（均P目的：探讨盐酸石蒜碱（lycorine hydrochloride，LH）对肝癌HCCLM3细胞恶性生物学行为的影响及其对circASH2L/ miR-124-3p轴的调控作用。方法：将HCCLM3细胞分为不同浓度LH处理组（LH-L、LH-M、LH-H组）和Con、si-NC、si-circASH2L、 LH+pcDNA、LH+pcDNA-circASH2L组；以 CCK-8法、平板克隆形成实验、流式细胞术、细胞划痕实验和 Transwell小室实验分别 检测HCCLM3细胞的增殖、克隆形成、凋亡、迁移和侵袭；qPCR法检测HCCLM3细胞中circASH2L和miR-124-3p的表达量；双荧 光素酶报告基因实验检测 circASH2L 和 miR-124-3p 的靶向关系；WB 法检测 cleaved-caspase3、cleaved-caspase9、E-cadherin、 N-cadherin蛋白表达量。结果：与Con组比较，LH不同浓度组细胞增殖抑制率升高（P<0.05），凋亡率与cleaved-caspase3、cleaved-caspase9、E-cadherin 蛋白水平升高（均P<0.05），miR-124-3p 的表达水平升高（P<0.05），克隆形成数与侵袭细胞数减少（均P<0.05），划痕愈合率与N-cadherin蛋白水平降低（P<0.05），circASH2L的表达水平降低（P<0.05），且不同浓度组间比较差异有统 计学意义（P<0.05）。circASH2L可负向调控 miR-124-3p。与 si-NC 组比较，si-circASH2L组细胞增殖抑制率升高（P<0.05），凋亡 率与 cleaved-caspase3、cleaved-caspase9、E-cadherin 蛋白水平升高（均 P<0.05），划痕愈合率与 N-cadherin 蛋白水平降低（均 P<0.05），克隆形成数与侵袭细胞数减少（均 P<0.05）。与 LH+pcDNA 组比较，LH+pcDNA-circASH2L 组 miR-124-3p的表达水平 降低（P<0.05），细胞增殖抑制率降低（P<0.05），凋亡率与 cleaved-caspase3、cleaved-caspase9、E-cadherin 蛋白水平降低（均 P<0.05），克隆形成数与侵袭细胞数增多（均 P<0.05），划痕愈合率与N-cadherin蛋白水平升高（均 P<0.05）。结论：LH可通过调控 circASH2L/miR-124-3p轴来抑制肝癌细胞HCCLM3的增殖、迁移、侵袭并诱导其凋亡。

Objective: To explore the effect of lycorine hydrochloride (LH) on the malignant biological behaviors of liver cancer HCCLM3 cells and its regulation on circASH2L/miR-124-3p axis. Methods: HCCLM3 cells treated with different concentrations of LH were divided into LH groups with different concentrations (LH-L group, LH-M group and LH-H group), Con group, si-NC group, si-circASH2L group, LH+pcDNA group, LH+pcDNA-circASH2L group. CCK-8 assay, plate clone formation test, flow cytometry, wound-healing assay, and Transwell assay were used to detect the proliferation, clone formation, apoptosis, migration and invasion of HCCLM3 cells. qPCR was used to detect the expression levels of circASH2L and miR-124-3p in HCCLM3 cells. Dual luciferase reporter gene assay was used to experiment detect the targeting relationship between circASH2L and miR-124-3p. WB was used to detect the expression of cleaved-caspase3, cleaved-caspase9, E-cadherin, and N-cadherin.Results: Compared with the Con group, the cell proliferation inhibition rates of LH groups with different concentrations were increased (P<0.05), the apoptosis rates and the expression levels of cleaved-caspase3, cleaved-caspase9 and E-cadherin were increased (all P<0.05), the expression levels of miR-124-3p were increased (P<0.05), the number of clone formation and invasive cells was decreased (all P<0.05), the wound-healing rates and the expression levels of N-cadherin were decreased (all P<0.05), and the expression levels of circASH2L were decreased (P<0.05), and the difference among different concentration groups was statistically significant(all P<0.05). CircASH2L could negatively regulate miR-124-3p. Compared with the si-NC group, the cell proliferation inhibition rates of the si-circASH2L group was increased (P<0.05), and the apoptosis rate and the expression levels of cleaved-caspase3, cleaved-caspase9 and E-cadherin were increased (all P<0.05), the wound-healing rate and the expression levels of N-cadherin were decreased (all P<0.05), and the number of clone formation and invasive cells was decreased (all P<0.05). Compared with the LH+pcDNA group, the expression level of miR-124-3p in the LH+ pcDNA-circASH2L group was decreased (P<0.05), the cell proliferation inhibition rate was decreased (P<0.05), the apoptosis rate and the expression levels of cleaved-caspase3, cleaved-caspase9 and E-cadherin were decreased (all P<0.05), the number of clone formation and invasive cells was increased (P<0.05), and the wound-healing rate and expression level of N-cadherin were increased (all P<0.05).

河南省科技攻关计划资助项目（No.162102310331）；河南省重点科技攻关计划资助项目（No.142102310128）